Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virusisolates

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cg.authorship.typesCGIAR and developing country instituteen_US
cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationQueensland University of Technologyen_US
cg.contributor.affiliationEthiopian Institute of Agricultural Researchen_US
cg.contributor.affiliationCentre for Pacific Crops and Trees, Fijien_US
cg.contributor.affiliationBotswana International University of Science and Technologyen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationInstitute of Molecular Biology and Biotechnology, Greeceen_US
cg.contributor.donorAfrica Biosciences Challenge Funden_US
cg.contributor.donorSwedish International Development Cooperation Agencyen_US
cg.contributor.donorDepartment for International Development, United Kingdomen_US
cg.contributor.donorBill & Melinda Gates Foundationen_US
cg.contributor.donorSyngenta Foundation for Sustainable Agricultureen_US
cg.contributor.donorDepartment of Foreign Affairs and Trade, Australiaen_US
cg.coverage.regionAfricaen_US
cg.coverage.regionEastern Africaen_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.1111/aab.12725en_US
cg.isijournalISI Journalen_US
cg.issn1744-7348en_US
cg.issue2en_US
cg.journalAnnals of Applied Biologyen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.ilriANIMAL DISEASESen_US
cg.subject.ilriRESEARCHen_US
cg.volume180en_US
dc.contributor.authorKidanemariam, Dawit B.en_US
dc.contributor.authorSukal, A.C.en_US
dc.contributor.authorAbraham, Adane D.en_US
dc.contributor.authorNjuguna, Joyce N.en_US
dc.contributor.authorStomeo, Francescaen_US
dc.contributor.authorDale, J.L.en_US
dc.contributor.authorJames, A.P.en_US
dc.contributor.authorHarding, R.M.en_US
dc.date.accessioned2022-08-01T14:33:26Zen_US
dc.date.available2022-08-01T14:33:26Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/120387en_US
dc.titleIncidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virusisolatesen_US
dcterms.abstractTaro (Colocasia esculenta) and tannia (Xanthosoma sp.) plants growing in 25 districts across Ethiopia, Kenya, Tanzania and Uganda were surveyed for four RNA viruses. Leaf samples from 392 plants were tested for cucumber mosaic virus (CMV), dasheen mosaic virus (DsMV), taro vein chlorosis virus (TaVCV) and Colocasia bobone disease‐associated virus (CBDaV) by RT‐PCR. No samples tested positive for TaVCV or CBDaV, while CMV was only detected in three tannia samples with mosaic symptoms from Uganda. DsMV was detected in 40 samples, including 36 out of 171 from Ethiopia, one out of 94 from Uganda and three out of 41 from Tanzania, while none of the 86 samples from Kenya tested positive for any of the four viruses. The complete genomes of nine DsMV isolates from East Africa were cloned and sequenced. Phylogenetic analyses based on the amino acid sequence of the DsMV CP‐coding region revealed two distinct clades. Isolates from Ethiopia were distributed in both clades, while samples from Uganda and Tanzania belong to different clades. Seven possible recombination events were identified from the analysis carried out on the available 15 full‐length DsMV isolates. Nucleotide substitution ratio analysis revealed that all the DsMV genes are under strong negative selection pressure.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceScientistsen_US
dcterms.available2021-09-07en_US
dcterms.bibliographicCitationKidanemariam, D.B., Sukal, A.C., Abraham, A.D., Njuguna, J.N., Stomeo, F., Dale, J.L., James, A.P. and Harding, R.M. 2022. Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virusisolates. Annals of Applied Biology 180(2): 211–223.en_US
dcterms.extentp. 211-223en_US
dcterms.issued2022-03en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.publisherWileyen_US
dcterms.subjectvirusesen_US
dcterms.subjecttaroen_US
dcterms.subjectxanthosoma sagittifoliumen_US
dcterms.subjectplant virusesen_US
dcterms.subjectcolocasia esculentaen_US
dcterms.typeJournal Articleen_US

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