Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virusisolates

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationQueensland University of Technologyen
cg.contributor.affiliationEthiopian Institute of Agricultural Researchen
cg.contributor.affiliationCentre for Pacific Crops and Trees, Fijien
cg.contributor.affiliationBotswana International University of Science and Technologyen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationInstitute of Molecular Biology and Biotechnology, Greeceen
cg.contributor.donorAfrica Biosciences Challenge Funden
cg.contributor.donorSwedish International Development Cooperation Agencyen
cg.contributor.donorDepartment for International Development, United Kingdomen
cg.contributor.donorBill & Melinda Gates Foundationen
cg.contributor.donorSyngenta Foundation for Sustainable Agricultureen
cg.contributor.donorDepartment of Foreign Affairs and Trade, Australiaen
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1111/aab.12725en
cg.isijournalISI Journalen
cg.issn1744-7348en
cg.issue2en
cg.journalAnnals of Applied Biologyen
cg.reviewStatusPeer Reviewen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriRESEARCHen
cg.volume180en
dc.contributor.authorKidanemariam, Dawit B.en
dc.contributor.authorSukal, A.C.en
dc.contributor.authorAbraham, Adane D.en
dc.contributor.authorNjuguna, Joyce N.en
dc.contributor.authorStomeo, Francescaen
dc.contributor.authorDale, J.L.en
dc.contributor.authorJames, A.P.en
dc.contributor.authorHarding, R.M.en
dc.date.accessioned2022-08-01T14:33:26Zen
dc.date.available2022-08-01T14:33:26Zen
dc.identifier.urihttps://hdl.handle.net/10568/120387
dc.titleIncidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virusisolatesen
dcterms.abstractTaro (Colocasia esculenta) and tannia (Xanthosoma sp.) plants growing in 25 districts across Ethiopia, Kenya, Tanzania and Uganda were surveyed for four RNA viruses. Leaf samples from 392 plants were tested for cucumber mosaic virus (CMV), dasheen mosaic virus (DsMV), taro vein chlorosis virus (TaVCV) and Colocasia bobone disease‐associated virus (CBDaV) by RT‐PCR. No samples tested positive for TaVCV or CBDaV, while CMV was only detected in three tannia samples with mosaic symptoms from Uganda. DsMV was detected in 40 samples, including 36 out of 171 from Ethiopia, one out of 94 from Uganda and three out of 41 from Tanzania, while none of the 86 samples from Kenya tested positive for any of the four viruses. The complete genomes of nine DsMV isolates from East Africa were cloned and sequenced. Phylogenetic analyses based on the amino acid sequence of the DsMV CP‐coding region revealed two distinct clades. Isolates from Ethiopia were distributed in both clades, while samples from Uganda and Tanzania belong to different clades. Seven possible recombination events were identified from the analysis carried out on the available 15 full‐length DsMV isolates. Nucleotide substitution ratio analysis revealed that all the DsMV genes are under strong negative selection pressure.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2021-09-07
dcterms.bibliographicCitationKidanemariam, D.B., Sukal, A.C., Abraham, A.D., Njuguna, J.N., Stomeo, F., Dale, J.L., James, A.P. and Harding, R.M. 2022. Incidence of RNA viruses infecting taro and tannia in East Africa and molecular characterisation of dasheen mosaic virusisolates. Annals of Applied Biology 180(2): 211–223.en
dcterms.extentp. 211-223en
dcterms.issued2022-03
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherWileyen
dcterms.subjectvirusesen
dcterms.subjecttaroen
dcterms.subjectxanthosoma sagittifoliumen
dcterms.subjectplant virusesen
dcterms.subjectcolocasia esculentaen
dcterms.typeJournal Article

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