Quantitative proteome analysis of bovine mammary gland reveals protein dynamic changes involved in peak and late lactation stages

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationChina Agricultural Universityen
cg.contributor.affiliationAgricultural Commission of Haian County, Chinaen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.creator.identifierXianrui Zheng: 0000-0002-1784-7393
cg.creator.identifierChao Ning: 0000-0001-8247-1700
cg.creator.identifierYichun Dong: 0000-0002-6850-3830
cg.creator.identifierPengju Zhao: 0000-0001-7357-808X
cg.creator.identifierJunhui Li: 0000-0001-5636-1348
cg.creator.identifierJiang Li: 0000-0001-6298-2036
cg.creator.identifierJian Feng Liu: 0000-0003-1589-9605
cg.creator.identifierRaphael Mrode: 0000-0003-1964-5653
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.bbrc.2017.10.038en
cg.issn0006-291Xen
cg.issue1-2en
cg.journalBiochemical and Biophysical Research Communicationsen
cg.subject.ilriCATTLEen
cg.subject.ilriDAIRYINGen
cg.volume494en
dc.contributor.authorZheng, Xianruien
dc.contributor.authorNing, Chaoen
dc.contributor.authorDong, Yichunen
dc.contributor.authorZhao, Pengjuen
dc.contributor.authorLi, Junhuien
dc.contributor.authorZiyao Fanen
dc.contributor.authorLi, Jiangen
dc.contributor.authorYing Yuen
dc.contributor.authorMrode, Raphael A.en
dc.contributor.authorLiu, Jian Fengen
dc.date.accessioned2017-10-13T19:05:49Zen
dc.date.available2017-10-13T19:05:49Zen
dc.identifier.urihttps://hdl.handle.net/10568/89005
dc.titleQuantitative proteome analysis of bovine mammary gland reveals protein dynamic changes involved in peak and late lactation stagesen
dcterms.abstractMammary gland is an important organ for milk synthesis and secretion. It undergoes dramatic physiological changes to adapt the shift from peak to late lactation stage. Protein plays a final very vital role in many life functions, and the protein changes during different lactation stages potentially reflect the biology of lactation and the functions of mammary gland in cows. In current study, we adopted tandem mass tags label-based quantitative analysis technique and to investigate proteome changes occurring in bovine mammary gland from peak to late lactation stages. A total of 3753 proteins from mammary tissues taken at two lactation points from four individual cows by biopsy were quantified, out of which 179 proteins were expressed differentially between two stages. We observed five new DEPs (PLIN2, LPIN1, PLIN3, AACS and DHCR7) and nine functional well-studies known proteins (PLIN2, LPIN1, PLIN3, GSN, CD74, MMP2, SOD1, SOD3 and GPX3) related to milk performance and mammary morphology. Bioinformatics analyses of the DEPs showed a majority of the up-regulated proteins during late lactation stage were related to apoptosis and immune process, while the downregulated proteins were mainly involved in localization, lipid metabolic and transport process. This suggests that the mammary gland can adapt to different molecular functions according to the biological need of the animal. From the integrated analysis of the differentially expressed proteins with known quantitative trait loci and genome-wide association study data, we identified 95 proteins may potentially affect milking performance. We expect findings in this study could be a valuable resource for future studies investigating the bovine proteome and functional studies.en
dcterms.accessRightsLimited Access
dcterms.audienceScientistsen
dcterms.bibliographicCitationXianrui Zheng, Chao Ning, Yichun Dong, Pengju Zhao, Junhui Li, Ziyao Fan, Jiang Li, Ying Yu, Mrode, R. and Jian-feng Liu. 2017. Quantitative proteome analysis of bovine mammary gland reveals protein dynamic changes involved in peak and late lactation stages. Biochemical and Biophysical Research Communications 494(1-2):292–297.en
dcterms.extentp. 292-297en
dcterms.issued2017-12
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherElsevieren
dcterms.subjectcattleen
dcterms.subjectcowsen
dcterms.subjectlactation stageen
dcterms.subjectmammary glandsen
dcterms.subjectmilken
dcterms.subjectproteinsen
dcterms.subjectsecretionen
dcterms.typeJournal Article

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