Mapping of QTL associated with resistance to Cassava Brown Streak and Mosaic Diseases in outcrossing cassava cultivars locally cultivated in Tanzania

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationSugarcane Research Institute, Tanzaniaen
cg.contributor.affiliationUniversity of Pretoriaen
cg.contributor.affiliationNaliendele Agricultural Research Institute, Tanzaniaen
cg.contributor.affiliationUkiriguru Agricultural Research Institute, Tanzaniaen
cg.contributor.affiliationUniversity of Californiaen
cg.contributor.affiliationDow AgroSciencesen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.affiliationUniversity of Arizonaen
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.donorBill & Melinda Gates Foundationen
cg.coverage.countryTanzania
cg.coverage.iso3166-alpha2TZ
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.howPublishedFormally Publisheden
cg.placeSan Diego, CAen
cg.subject.iitaGENETIC IMPROVEMENTen
cg.subject.iitaPLANT DISEASESen
cg.subject.iitaPLANT GENETIC RESOURCESen
dc.contributor.authorMasumba, E.en
dc.contributor.authorMerwe, A. van deren
dc.contributor.authorMyburg, Z.en
dc.contributor.authorKapinga, F.en
dc.contributor.authorKasele, S.en
dc.contributor.authorKulembeka, H.P.en
dc.contributor.authorMkamilo, G.S.en
dc.contributor.authorRounsley, S.en
dc.contributor.authorProchnik, S.E.en
dc.contributor.authorKanju, E.en
dc.contributor.authorFerguson, M.en
dc.date.accessioned2017-02-02T09:06:17Zen
dc.date.available2017-02-02T09:06:17Zen
dc.identifier.urihttps://hdl.handle.net/10568/79463
dc.titleMapping of QTL associated with resistance to Cassava Brown Streak and Mosaic Diseases in outcrossing cassava cultivars locally cultivated in Tanzaniaen
dcterms.abstractCassava brown streak (CBSD) and mosaic (CMD) diseases are the major cassava production constraints in the East African region. Efforts to control these diseases were initiated in northeastern Tanganyika in the 1930’s using conventional breeding methods. Despite these efforts, CBSD is spreading, threatening central and western Africa. Identification of molecular markers associated with observed plant resistance and tolerance would facilitate marker assisted breeding leading to efficient breeding, knowledge-based deployment of resistance genes and pre-emptive breeding in West Africa. QTL analysis using a bi-parental F1 mapping population was undertaken to identify QTL for CMD resistance in the variety ‘Albert’ and CBSD resistance in the variety ‘Namikonga’. A one-step genetic linkage map composed of 986 SNP markers and 18 linkage groups spanning 1826.3 cM was generated and used to create a framework map of 242 loci. Phenotyping data was obtained from two disease hotspots in Tanzania from 226 F1 progeny. Significant QTL were identified on chromosomes VIII, XV and I for CMD, CBSD foliar and root symptoms respectively. The maximum LOD score of 19.32 explained variation 34.9% of variation for CMD resistance. CBSD foliar and root symptoms revealed different QTL with moderate LOD scores of up to 5.09 and 7.33, explaining 10.7 and 16.2% respectively of variation. Several other minor significant QTL for all traits were also identified. Genes within QTL regions are being characterized.en
dcterms.accessRightsLimited Access
dcterms.audienceScientistsen
dcterms.bibliographicCitationMasumba, E., van der Merwe, A., Myburg, Z., Kapinga, F., Kasele, S., Kulembeka, H., ... & M. Ferguson, M. (2015). Mapping of QTL associated with resistance to Cassava Brown Streak and Mosaic Diseases in outcrossing cassava cultivars locally cultivated in Tanzania. Poster session at: Plant & Animal Genome XXIII, January 10-14, San Diego, CAen
dcterms.issued2015-01
dcterms.languageen
dcterms.publisherPlant and Animal Genomesen
dcterms.subjectcassavaen
dcterms.subjectplant virusesen
dcterms.subjectafrican cassava mosaic virusen
dcterms.subjectbrown streak virusen
dcterms.subjectphenotypingen
dcterms.typePoster

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