Candidate genes for field resistance to cassava brown streak disease revealed through the analysis of multiple data sources

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.affiliationNaliendele Agricultural Research Instituteen
cg.contributor.affiliationUniversity of Californiaen
cg.contributor.affiliationSeeds & Traits R&D, USAen
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.donorBill & Melinda Gates Foundationen
cg.contributor.donorCGIAR Trust Funden
cg.contributor.initiativeAccelerated Breeding
cg.coverage.countryTanzania
cg.coverage.iso3166-alpha2TZ
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierMorag Ferguson: 0000-0002-7763-5173
cg.creator.identifierRodney Eyles: 0000-0002-8138-2180
cg.creator.identifierGarcia-Oliveira AL: 0000-0001-8561-4172
cg.creator.identifierTrushar Shah: 0000-0002-0091-7981
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.3389/fpls.2023.1270963en
cg.identifier.iitathemeBIOTECH & PLANT BREEDING
cg.isijournalISI Journalen
cg.issn1664-462Xen
cg.issue1270963en
cg.journalFrontiers in Plant Scienceen
cg.reviewStatusPeer Reviewen
cg.subject.actionAreaGenetic Innovation
cg.subject.iitaAGRONOMYen
cg.subject.iitaCASSAVAen
cg.subject.iitaDISEASE CONTROLen
cg.subject.iitaFOOD SECURITYen
cg.subject.iitaGENETIC IMPROVEMENTen
cg.subject.iitaPLANT BREEDINGen
cg.subject.iitaPLANT GENETIC RESOURCESen
cg.subject.iitaPLANT HEALTHen
cg.subject.iitaPLANT PRODUCTIONen
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 2 - Zero hungeren
cg.volume14en
dc.contributor.authorFerguson, M.en
dc.contributor.authorEyles, R.P.en
dc.contributor.authorGarcia-Oliveira, A.L.en
dc.contributor.authorKapinga, F.en
dc.contributor.authorMasumba, E.A.en
dc.contributor.authorAmuge, T.en
dc.contributor.authorBredeson, J.V.en
dc.contributor.authorRokhsar, D.S.en
dc.contributor.authorLyons, J.B.en
dc.contributor.authorShah, T.en
dc.contributor.authorRounsley, S.en
dc.contributor.authorMkamilo, G.en
dc.date.accessioned2023-11-09T14:23:32Zen
dc.date.available2023-11-09T14:23:32Zen
dc.identifier.urihttps://hdl.handle.net/10568/132877
dc.titleCandidate genes for field resistance to cassava brown streak disease revealed through the analysis of multiple data sourcesen
dcterms.abstractCassava (Manihot esculenta Crantz) is a food and industrial storage root crop with substantial potential to contribute to managing risk associated with climate change due to its inherent resilience and in providing a biodegradable option in manufacturing. In Africa, cassava production is challenged by two viral diseases, cassava brown streak disease (CBSD) and cassava mosaic disease. Here we detect quantitative trait loci (QTL) associated with CBSD in a biparental mapping population of a Tanzanian landrace, Nachinyaya and AR37-80, phenotyped in two locations over three years. The purpose was to use the information to ultimately facilitate either marker-assisted selection or adjust weightings in genomic selection to increase the efficiency of breeding. Results from this study were considered in relation to those from four other biparental populations, of similar genetic backgrounds, that were phenotyped and genotyped simultaneously. Further, we investigated the co-localization of QTL for CBSD resistance across populations and the genetic relationships of parents based on whole genome sequence information. Two QTL on chromosome 4 for resistance to CBSD foliar symptoms and one on each of chromosomes 11 and 18 for root necrosis were of interest. Of significance within the candidate genes underlying the QTL on chromosome 4 are Phenylalanine ammonia-lyase (PAL) and Cinnamoyl-CoA reductase (CCR) genes and three PEPR1-related kinases associated with the lignin pathway. In addition, a CCR gene was also underlying the root necrosis-resistant QTL on chromosome 11. Upregulation of key genes in the cassava lignification pathway from an earlier transcriptome study, including PAL and CCR, in a CBSD-resistant landrace compared to a susceptible landrace suggests a higher level of basal lignin deposition in the CBSD-resistant landrace. Earlier RNAscope® in situ hybridisation imaging experiments demonstrate that cassava brown streak virus (CBSV) is restricted to phloem vessels in CBSV-resistant varieties, and phloem unloading for replication in mesophyll cells is prevented. The results provide evidence for the involvement of the lignin pathway. In addition, five eukaryotic initiation factor (eIF) genes associated with plant virus resistance were found within the priority QTL regions.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2023-11-03
dcterms.bibliographicCitationFerguson, M., Eyles, R.P., Garcia-Oliveira, A.L., Kapinga, F., Masumba, E.A., Amuge, T., ... & Mkamilo, G. (2023). Candidate genes for field resistance to cassava brown streak disease revealed through the analysis of multiple data sources. Frontiers in Plant Science, 14: 1270963, 1-16.en
dcterms.descriptionOpen Access Journalen
dcterms.extent1-16en
dcterms.issued2023
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherFrontiersen
dcterms.subjectligninsen
dcterms.subjectphenylalanine ammonia lyaseen
dcterms.subjectquantitative trait locien
dcterms.subjectcassavaen
dcterms.subjectafrican cassava mosaic virusen
dcterms.subjectfood securityen
dcterms.typeJournal Article

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