Morphophysiological diversity of rhizobia nodulating pigeon pea (Cajanus cajan L. Millsp.) growing in Ethiopia

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationAdama Science and Technology Universityen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationHawassa Universityen
cg.contributor.crpLivestock
cg.coverage.countryEthiopia
cg.coverage.iso3166-alpha2ET
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierEndalkachew Wolde-meskel: 0000-0001-6433-0162en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.5897/ajb2017.16338en
cg.issn1684-5315en
cg.issue6en
cg.journalAfrican Journal of Biotechnologyen
cg.reviewStatusPeer Reviewen
cg.subject.ilriFEEDSen
cg.subject.ilriRESEARCHen
cg.subject.ilriLEGUMESen
cg.volume17en
dc.contributor.authorDegefu, T.en
dc.contributor.authorWoldemeskel, Endalkachewen
dc.contributor.authorAdem, M.en
dc.contributor.authorFikre, Asnakeen
dc.contributor.authorAmede, Tilahunen
dc.contributor.authorOjiewo, Christopher Ochiengen
dc.date.accessioned2018-03-20T13:41:34Zen
dc.date.available2018-03-20T13:41:34Zen
dc.identifier.urihttps://hdl.handle.net/10568/91713
dc.titleMorphophysiological diversity of rhizobia nodulating pigeon pea (Cajanus cajan L. Millsp.) growing in Ethiopiaen
dcterms.abstractigeon pea (Cajanus cajan (L.) Millsp.) is an important protein source grown in several tropical and sub-tropical countries, and is considered a multi-purpose plant that is resistant to the conditions where drought and salinity is a common phenomenon. The aim of this study was to evaluate the diversity of rhizobial isolates obtained from root nodules of pigeon pea plants grown in central and southern Ethiopia. A total of 116 nitrogen-fixing rhizobial strains were isolated. The bacterial isolates were characterized by 91 phenotypic traits including cultural characteristics, intrinsic antibiotic and heavy metal resistance, salt, pH and incubation temperature tolerance, and carbon and nitrogen sources utilization ability. Preliminary symbiotic properties of the isolates were also evaluated. The isolates were compared with seven reference strains of rhizobia by application of the unweighted pair group method with arithmetic means (UPGMA) using NTSYSpc Version 2.1 software program. The dendrogram constructed from cluster analysis of 91 phenotypic traits, grouped them into six clusters and eight un-clustered positions at 80% relative similarity. Cluster I contained 83% of the test isolates that were grouped together with the reference strains Bradyrhizobium japonicum (HAMBI 2314T) and Bradyrhizobium elkanii (LMG 6164), suggesting that pigeon pea is commonly nodulated by bradyrhizobia. Results from symbiotic effectiveness test revealed that majority of the isolates were found to be effective. Generally, this investigation demonstrated that rhizobial population nodulating C. cajan on the study area were phenotypically diverse and symbiotically effective. Furthermore, the result indicates the existence of strains in the collection, which can tolerate environmental stresses, thus can be developed into inoculant for pigeon pea inoculation and production in Ethiopia and beyond.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2018-02-07en
dcterms.bibliographicCitationDegefu, T., Wolde-meskel, E., Adem, M., Fikre, A., Amede, T. and Ojiewo, C. 2018. Morphophysiological diversity of rhizobia nodulating pigeon pea (Cajanus cajan L. Millsp.) growing in Ethiopia. African Journal of Biotechnology 17(6):167-177.en
dcterms.extentp. 167-177en
dcterms.issued2018-02-15en
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherAcademic Journalsen
dcterms.subjectplantsen
dcterms.subjectpigeon peasen
dcterms.subjectresearchen
dcterms.subjectbradyrhizobiumen
dcterms.subjectcajanus cajanen
dcterms.subjectrhizobiumen
dcterms.typeJournal Article

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