Identification of resistanceassociated proteins in closelyrelated maize lines varying in aflatoxin accumulation

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cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationLouisiana State Universityen
cg.contributor.affiliationUnited States Department of Agricultureen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.crpMaize
cg.contributor.donorUnited States Department of Agricultureen
cg.coverage.countryNigeria
cg.coverage.iso3166-alpha2NG
cg.coverage.regionAfrica
cg.coverage.regionWestern Africa
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1007/s11032-011-9597-3en
cg.isijournalISI Journalen
cg.issn1380-3743en
cg.issue1en
cg.journalMolecular Breedingen
cg.reviewStatusPeer Reviewen
cg.subject.iitaMAIZEen
cg.subject.iitaAFLATOXINen
cg.volume30en
dc.contributor.authorChen, Z.en
dc.contributor.authorBrown, R.L.en
dc.contributor.authorMenkir, A.en
dc.contributor.authorCleveland, T.E.en
dc.date.accessioned2016-10-27T08:30:38Zen
dc.date.available2016-10-27T08:30:38Zen
dc.identifier.urihttps://hdl.handle.net/10568/77432
dc.titleIdentification of resistanceassociated proteins in closelyrelated maize lines varying in aflatoxin accumulationen
dcterms.abstractAspergillus flavus infection of maize and subsequent contamination with carcinogenic aflatoxins poses serious health concerns, especially in developing countries. Maize lines resistant to A. flavus infection have been identified; however, the development of commercially-useful aflatoxin-resistant maize lines has been hindered due to a lack of breeding markers. To identify maize resistance-associated proteins (RAPs) as potential markers for breeding, 52 BC1S4 lines developed from crosses between five African maize inbreds and five temperate aflatoxin-resistant lines were screened using the kernel screening assay. Five pairs of closely-related lines that had 75–94% genetic similarity within each pair and which varied within each pair in aflatoxin accumulation were selected for proteomic investigation. Kernel embryo and endosperm protein profile differences within the pair and across pairs were compared using two-dimensional polyacrylamide gel electrophoresis. Differentially expressed (≥1.5-fold) RAPs were sequenced through tandem mass spectrometry and were identified as antifungal, stress-related, storage or regulatory proteins. Sequence homology analysis highlighted several proteins in maize that confer resistance to A. flavus infection and/or aflatoxin production.en
dcterms.accessRightsLimited Access
dcterms.available2011-06-22
dcterms.bibliographicCitationChen, Z., Brown, R.L, Menkir, A. & Cleveland, T.E. (2012). Identification of resistance-associated proteins in closely-related maize lines varying in aflatoxin accumulation. Molecular Breeding, 30(1), 53-68.en
dcterms.extent53-68en
dcterms.issued2012-06
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherSpringeren
dcterms.subjectaflatoxinsen
dcterms.subjectproteinsen
dcterms.subjectmaizeen
dcterms.subjectaspergillus flavusen
dcterms.subjectantifungal propertiesen
dcterms.subjectgel electrophoresisen
dcterms.typeJournal Article

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