Rapid and efficient production of transgenic East African highland banana (Musa spp.) using intercalary meristematic tissues

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.affiliationKawanda Agricultural Research Institute, Ugandaen
cg.coverage.countryUganda
cg.coverage.iso3166-alpha2UG
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.issn1684-5315en
cg.issue10en
cg.reviewStatusPeer Reviewen
cg.subject.iitaSMALLHOLDER FARMERSen
cg.subject.iitaBANANAen
cg.subject.iitaPESTS OF PLANTSen
cg.subject.iitaPLANT PRODUCTIONen
cg.subject.iitaFOOD SECURITYen
cg.subject.iitaPLANT GENETIC RESOURCESen
cg.subject.iitaHANDLING, TRANSPORT, STORAGE AND PROTECTION OF AGRICULTURAL PRODUCTSen
cg.subject.iitaLIVELIHOODSen
cg.subject.iitaDISEASE CONTROLen
cg.subject.iitaNUTRITIONen
cg.subject.iitaGENETIC IMPROVEMENTen
cg.subject.iitaPLANT DISEASESen
cg.volume7en
dc.contributor.authorTripathi, L.en
dc.contributor.authorTripathi, J.N.en
dc.contributor.authorTushemereirwe, Wilberforce K.en
dc.date.accessioned2018-02-06T12:15:16Zen
dc.date.available2018-02-06T12:15:16Zen
dc.identifier.urihttps://hdl.handle.net/10568/90891
dc.titleRapid and efficient production of transgenic East African highland banana (Musa spp.) using intercalary meristematic tissuesen
dcterms.abstractEast Africa is the largest banana producing and consuming region in Africa. In particular, the East African Highland Banana serves as the major staple crop of countries like Uganda, but production is constrained by a number of serious pests and diseases. Banana breeding is a very difficult and slow process, so genetic engineering offers an alternative approach to improvement. A transformation system using intercalary meristematic tissues was developed using Agrobacterium strain EHA105 harboring the binary vector pCAMBIA2301 containing the gusA reporter gene and nptII as selectable marker. In this paper, a new transformation protocol is described that yields kanamycin-resistant, GUSexpressing banana plants from roughly 10% of the initial explants. The resulting fully-rooted transgenic plants do not appear to be chimeras since they can be stably propagated, GUS activity is observed uniformly throughout the plants including the germline cells of the meristem, and PCR and Southern blots indicate stable integration of the genes into the genome.en
dcterms.accessRightsOpen Access
dcterms.bibliographicCitationTripathi, L., Tripathi, J.N. & Tushemereirwe, W.K. (2008). Rapid and efficient production of transgenic East African Highland Banana (Musa spp.) using intercalary meristematic tissues. African Journal of Biotechnology, 7(10), 1438-1445.en
dcterms.extentp. 1438-1445en
dcterms.issued2008-05
dcterms.languageen
dcterms.subjectagrobacteriumen
dcterms.subjectintercalary meristemen
dcterms.subjectgenetic transformationen
dcterms.subjectbananasen
dcterms.subjecteast african highland bananasen
dcterms.subjectxanthomonasen
dcterms.subjectbanana streak virusen
dcterms.typeJournal Article

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