Artificial microRNA-derived resistance to Cassava brown streak disease

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cg.contributor.affiliationNational Crops Resources Research Institute, Ugandaen
cg.contributor.affiliationMakerere Universityen
cg.contributor.affiliationIndian Council of Agricultural Researchen
cg.contributor.affiliationInternational Center for Tropical Agricultureen
cg.contributor.affiliationDonald Danforth Plant Science Centeren
cg.contributor.crpRoots, Tubers and Bananas
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.jviromet.2016.02.004en
cg.isijournalISI Journalen
cg.issn0166-0934en
cg.journalJournal of Virological Methodsen
cg.reviewStatusPeer Reviewen
cg.subject.ciatCASSAVAen
cg.subject.ciatPESTS AND DISEASESen
cg.volume231en
dc.contributor.authorWagaba, Henryen
dc.contributor.authorPatil, Basavaprabhu L.en
dc.contributor.authorMukasa, Settumbaen
dc.contributor.authorAlicai, Titusen
dc.contributor.authorFauquet, Claude M.en
dc.contributor.authorTaylor, Nigel J.en
dc.date.accessioned2016-03-28T20:23:26Zen
dc.date.available2016-03-28T20:23:26Zen
dc.identifier.urihttps://hdl.handle.net/10568/72739
dc.titleArtificial microRNA-derived resistance to Cassava brown streak diseaseen
dcterms.abstractArtificial miRNAs (amiRNA) were generated targeting conserved sequences within the genomes of the two causal agents of Cassava brown streak disease (CBSD): Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Transient expression studies on ten amiRNAs targeting 21 nt conserved sequences of P1(CBSV and UCBSV), P3(CBSV and UCBSV), CI(UCBSV), NIb(CBSV and UCBSV), CP(UCBSV) and the un-translated region (3′-UTR) were tested in Nicotiana benthamiana. Four out of the ten amiRNAs expressed the corresponding amiRNA at high levels. Transgenic N. benthamiana plants were developed for the four amiRNAs targeting the P1 and NIb genes of CBSV and the P1 and CP genes of UCBSV and shown to accumulate miRNA products. Transgenic plants challenged with CBSV and UCBSV isolates showed resistance levels that ranged between ∼20–60% against CBSV and UCBSV and correlated with expression levels of the transgenically derived miRNAs. MicroRNAs targeting P1 and NIb of CBSV showed protection against CBSV and UCBSV, while amiRNAs targeting the P1 and CP of UCBSV showed protection against UCBSV but were less efficient against CBSV. These results indicate a potential application of amiRNAs for engineering resistance to CBSD-causing viruses in cassava.en
dcterms.accessRightsOpen Access
dcterms.bibliographicCitationWagaba, Henry; Patil, Basavaprabhu L.; Mukasa, Settumba; Alicai, Titus; Fauquet, Claude M.; Taylor, Nigel J.. 2016. Artificial microRNA-derived resistance to Cassava brown streak disease. Journal of Virological Methods 231: 38-43.en
dcterms.extent231: 38-43en
dcterms.issued2016-05
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherElsevieren
dcterms.subjectcassava brown streak diseaseen
dcterms.subjectcassava brown streak virusen
dcterms.subjectmanihot esculentaen
dcterms.subjectplant virusesen
dcterms.subjectrna sequenceen
dcterms.subjectvirusesen
dcterms.subjectgenomesen
dcterms.subjectdisease controlen
dcterms.subjectresistanceen
dcterms.subjectvirus de las plantasen
dcterms.subjectsecuencia de arnen
dcterms.subjectvirusen
dcterms.subjectgenomasen
dcterms.subjectcassavaen
dcterms.subjectcontrol de enfermedadesen
dcterms.subjectresistenciaen
dcterms.typeJournal Article

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