Sequence-Based Genotyping of Expressed Swine Leukocyte Antigen Class I Alleles by Next-Generation Sequencing Reveal Novel Swine Leukocyte Antigen Class I Haplotypes and Alleles in Belgian, Danish, and Kenyan Fattening Pigs and Göttingen Minipigs

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cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationTechnical University of Denmarken
cg.contributor.donorMinistry of Higher Education and Science, Denmarken
cg.contributor.donorFederal Ministry for Economic Cooperation and Development, Germanyen
cg.creator.identifierRichard Bishop: 0000-0002-3720-9970
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.3389/fimmu.2017.00701en
cg.isijournalISI Journalen
cg.issn1664-3224en
cg.journalFrontiers in immunologyen
cg.reviewStatusPeer Reviewen
cg.subject.ilriPIGSen
cg.subject.ilriANIMAL DISEASESen
cg.volume8en
dc.contributor.authorSørensen, Maria Rathmannen
dc.contributor.authorIlsøe, Metteen
dc.contributor.authorStrube, Mikael Lenzen
dc.contributor.authorBishop, Richard P.en
dc.contributor.authorErbs, Gitteen
dc.contributor.authorHartmann, Sofie Bruunen
dc.contributor.authorJungersen, Gregersen
dc.date.accessioned2023-01-07T13:21:55Zen
dc.date.available2023-01-07T13:21:55Zen
dc.identifier.urihttps://hdl.handle.net/10568/126677
dc.titleSequence-Based Genotyping of Expressed Swine Leukocyte Antigen Class I Alleles by Next-Generation Sequencing Reveal Novel Swine Leukocyte Antigen Class I Haplotypes and Alleles in Belgian, Danish, and Kenyan Fattening Pigs and Göttingen Minipigsen
dcterms.abstractThe need for typing of the swine leukocyte antigen (SLA) is increasing with the expanded use of pigs as models for human diseases and organ-transplantation experiments, their use in infection studies, and for design of veterinary vaccines. Knowledge of SLA sequences is furthermore a prerequisite for the prediction of epitope binding in pigs. The low number of known SLA class I alleles and the limited knowledge of their prevalence in different pig breeds emphasizes the need for efficient SLA typing methods. This study utilizes an SLA class I-typing method based on next-generation sequencing of barcoded PCR amplicons. The amplicons were generated with universal primers and predicted to resolve 68-88% of all known SLA class I alleles dependent on amplicon size. We analyzed the SLA profiles of 72 pigs from four different pig populations; Göttingen minipigs and Belgian, Kenyan, and Danish fattening pigs. We identified 67 alleles, nine previously described haplotypes and 15 novel haplotypes. The highest variation in SLA class I profiles was observed in the Danish pigs and the lowest among the Göttingen minipig population, which also have the highest percentage of homozygote individuals. Highlighting the fact that there are still numerous unknown SLA class I alleles to be discovered, a total of 12 novel SLA class I alleles were identified. Overall, we present new information about known and novel alleles and haplotypes and their prevalence in the tested pig populations.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2017-06-16
dcterms.bibliographicCitationSørensen MR, Ilsøe M, Strube ML, Bishop R, Erbs G, Hartmann SB and Jungersen G (2017) Sequence-Based Genotyping of Expressed Swine Leukocyte Antigen Class I Alleles by Next-Generation Sequencing Reveal Novel Swine Leukocyte Antigen Class I Haplotypes and Alleles in Belgian, Danish, and Kenyan Fattening Pigs and Göttingen Minipigs. Front. Immunol. 8:701en
dcterms.issued2017-06
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherFrontiers Mediaen
dcterms.subjectpigsen
dcterms.typeJournal Article

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