Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars
| cg.authorship.types | CGIAR and developing country institute | en |
| cg.contributor.affiliation | Quaid-i-Azam University | en |
| cg.contributor.affiliation | Bahauddin Zakariya University | en |
| cg.contributor.affiliation | National Agricultural Research Centre, Pakistan | en |
| cg.contributor.affiliation | Chinese Academy of Agricultural Sciences | en |
| cg.contributor.affiliation | International Maize and Wheat Improvement Center | en |
| cg.contributor.donor | National Science Foundation of China | en |
| cg.contributor.donor | CGIAR Trust Fund | en |
| cg.contributor.donor | Hainan Yazhou Bay Seed Laboratory | en |
| cg.contributor.initiative | Accelerated Breeding | |
| cg.creator.identifier | awais rasheed: 0000-0003-2528-708X | |
| cg.creator.identifier | Huihui Li: 0000-0002-9117-5011 | |
| cg.howPublished | Formally Published | en |
| cg.identifier.doi | https://doi.org/10.3389/fpls.2022.972481 | en |
| cg.isijournal | ISI Journal | en |
| cg.issn | 1664-462X | en |
| cg.journal | Frontiers in Plant Science | en |
| cg.reviewStatus | Peer Review | en |
| cg.subject.actionArea | Genetic Innovation | |
| cg.subject.impactArea | Nutrition, health and food security | |
| cg.subject.impactArea | Climate adaptation and mitigation | |
| cg.volume | 13 | en |
| dc.contributor.author | Khan, Muhammad Ibrar | en |
| dc.contributor.author | Kainat, Zarnishal | en |
| dc.contributor.author | Maqbool, Saman | en |
| dc.contributor.author | Mehwish, Ambreen | en |
| dc.contributor.author | Ahmad, Suhaib | en |
| dc.contributor.author | Hafiz Muhammad Suleman | en |
| dc.contributor.author | Mahmood, Zahid | en |
| dc.contributor.author | Ali, Mohsin | en |
| dc.contributor.author | Aziz, Abdul | en |
| dc.contributor.author | Rasheed, Awais | en |
| dc.contributor.author | Huihui Li | en |
| dc.date.accessioned | 2023-01-25T20:02:44Z | en |
| dc.date.available | 2023-01-25T20:02:44Z | en |
| dc.identifier.uri | https://hdl.handle.net/10568/128239 | |
| dc.title | Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars | en |
| dcterms.abstract | Increasing global temperature has adverse effects on crop health and productivity at both seedling and reproductivity stages. It is paramount to develop heat tolerant wheat cultivars able to sustain under high and fluctuating temperature conditions. An experiment was conducted to characterize 194 historical wheat cultivars of Pakistan under high temperature at seedling stage to identify loci associated with heat tolerance using genome-wide association studies (GWAS). A quantitative trait locus, TaHST1, on chr4A was also characterized to identify the haplotypes at this locus associated with heat tolerance in wheat from Pakistan. Initially, the diversity panel was planted under control conditions (25°C/20°C day and night temperature) in a glass house. At three leaf stage, plants were subjected to heat stress (HS) by increasing temperature (40°C/35°C day and night), while one treatment was kept at control condition. After 7 days of HS, data were collected for seedling morphology. Heat stress reduced these traits by 25% (root weight) to 40% (shoot weight), and shoot biomass was largely affected by heat stress. A GWAS model, fixed and random model circulating probability unification (FarmCPU), identified 43 quantitative trait nucleotides (QTNs) on all chromosomes, except chr7B, were associated under both HS and control conditions. Thirteen QTNs were identified in control, while 30 QTNs were identified in HS condition. In total, 24 haplotypes were identified at TaHST1 locus, and most of the heat tolerant genotypes were assigned to Hap-20 and Hap-21. Eleven QTNs were identified within 0.3–3.1 Mb proximity of heat shock protein (HSP). Conclusively, this study provided a detailed genetic framework of heat tolerance in wheat at the seedling stage and identify potential genetic regions associated with heat tolerance which can be used for marker assisted selection (MAS) in breeding for heat stress tolerance. | en |
| dcterms.accessRights | Open Access | |
| dcterms.audience | Scientists | en |
| dcterms.available | 2022-08-25 | |
| dcterms.bibliographicCitation | Khan, M. I., Kainat, Z., Maqbool, S., Mehwish, A., Ahmad, S., Suleman, H. M., Mahmood, Z., Ali, M., Aziz, A., Rasheed, A., & Li, H. (2022). Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.972481 | en |
| dcterms.issued | 2022-08-25 | |
| dcterms.language | en | |
| dcterms.license | CC-BY-4.0 | |
| dcterms.publisher | Frontiers Media | en |
| dcterms.subject | heat stress | en |
| dcterms.subject | quantitative trait loci | en |
| dcterms.subject | nucleotides | en |
| dcterms.subject | single nucleotide polymorphism | en |
| dcterms.subject | spring wheat | en |
| dcterms.type | Journal Article |