Mapping EST-derived SSRs and ESTs involved in resistance to bacterial blight in Manihot esculenta

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cg.coverage.regionAfrica
cg.coverage.regionLatin America
cg.creator.identifierJoe Tohme: 0000-0003-2765-7101
cg.identifier.doihttps://doi.org/10.1139/g07-087en
cg.isijournalISI Journalen
cg.issn1480-3321en
cg.issue12en
cg.journalGenomeen
cg.reviewStatusPeer Reviewen
cg.subject.ciatCASSAVAen
cg.subject.ciatGENETIC RESOURCESen
cg.subject.ciatPESTS AND DISEASESen
cg.volume50en
dc.contributor.authorLópez, C.E.en
dc.contributor.authorQuesada Ocampo, LMen
dc.contributor.authorBohórquez Cháux, Adrianaen
dc.contributor.authorDuque E., Myriam Cristinaen
dc.contributor.authorVargas Daza, Jaimeen
dc.contributor.authorTohme, Joseph M.en
dc.contributor.authorVerdier, Valerie M.en
dc.date.accessioned2014-10-02T08:32:48Zen
dc.date.available2014-10-02T08:32:48Zen
dc.identifier.urihttps://hdl.handle.net/10568/43838
dc.titleMapping EST-derived SSRs and ESTs involved in resistance to bacterial blight in Manihot esculentaen
dcterms.abstractCassava (Manihot esculenta Crantz) is a major root crop widely grown in the tropics. Cassava bacterial blight, caused by Xanthomonas axonopodis pv. manihotis (Xam), is an important disease in Latin America and Africa resulting in significant losses. The preferred control method is the use of resistant genotypes. Mapping expressed sequence tags (ESTs) and determining their co-localization with quantitative trait loci (QTLs) may give additional evidence of the role of the corresponding genes in resistance or defense. Twenty-one EST-derived simple sequence repeats (SSRs) were mapped in 16 linkage groups. ESTs showing similarities with candidate resistance genes or defense genes were also mapped using strategies such as restriction fragment length polymorphisms, cleaved amplified polymorphic sequences, and allele-specific primers. In total, 10 defense-related genes and 2 bacterial artificial chromosomes (BACs) containing resistance gene candidates (RGCs) were mapped in 11 linkage groups. Two new QTLs associated with resistance to Xam strains CIO121 and CIO151 were detected in linkage groups A and U, respectively. The QTL in linkage group U explained 61.6% of the phenotypic variance and was associated with an RGC-containing BAC. No correlation was found between the new EST-derived SSRs or other mapped ESTs and the new or previously reported QTLs.en
dcterms.accessRightsOpen Access
dcterms.extentpp. 1078-1088en
dcterms.issued2007-12
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherCanadian Science Publishingen
dcterms.subjectmanihot esculentaen
dcterms.subjectchromosome mappingen
dcterms.subjectquantitative trait locien
dcterms.subjectgenetic markersen
dcterms.subjectgenetic resistanceen
dcterms.subjectcassava bacterial blighten
dcterms.subjectmapeo de cromosomasen
dcterms.subjectloci de rasgos cuantitativosen
dcterms.subjectmarcadores genéticosen
dcterms.subjectresistencia genéticaen
dcterms.subjectanublo bacteriano de la yucaen
dcterms.typeJournal Article

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