Agromorphologic, genetic and methylation profiling of Dioscorea and Musa species multiplied under three micropropagation systems

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.affiliationUniversity of Ibadanen
cg.contributor.crpMaize
cg.contributor.crpPolicies, Institutions, and Markets
cg.contributor.crpRoots, Tubers and Bananas
cg.coverage.countryNigeria
cg.coverage.iso3166-alpha2NG
cg.coverage.regionAfrica
cg.coverage.regionWestern Africa
cg.creator.identifierBadara GUEYE: 0000-0003-3157-8772
cg.creator.identifierRanjana Bhattacharjee: 0000-0002-5184-5930
cg.creator.identifierTrushar Shah: 0000-0002-0091-7981
cg.creator.identifierMichael Abberton: 0000-0003-2555-9591
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1371/journal.pone.0216717en
cg.identifier.iitathemeBIOMETRICS
cg.identifier.iitathemeBIOTECH & PLANT BREEDING
cg.isijournalISI Journalen
cg.issn1932-6203en
cg.issue5en
cg.journalPLOS ONEen
cg.reviewStatusPeer Reviewen
cg.subject.iitaBIOMETRICSen
cg.subject.iitaGENETIC IMPROVEMENTen
cg.subject.iitaPLANT BREEDINGen
cg.volume14en
dc.contributor.authorJekayinoluwa, T.en
dc.contributor.authorGueye, B.en
dc.contributor.authorBhattacharjee, Ranjanaen
dc.contributor.authorOsibanjo, O.en
dc.contributor.authorShah, T.en
dc.contributor.authorAbberton, Michael T.en
dc.date.accessioned2019-05-22T11:46:34Zen
dc.date.available2019-05-22T11:46:34Zen
dc.identifier.urihttps://hdl.handle.net/10568/101343
dc.titleAgromorphologic, genetic and methylation profiling of Dioscorea and Musa species multiplied under three micropropagation systemsen
dcterms.abstractPlant in vitro vegetative propagation using classical semi-solid culture medium is limited due to the low degree of automation, suboptimal nutrient availability and induced physiological stress which often reduce its efficiency. Temporary Immersion System (TIS) emerged as an innovative approach to optimize and eliminate the drawbacks associated with the conventional system of micropropagation. In this study, both Dioscorea and Musa spp. were subjected to conventional semi-solid culture media, complete immersion in shaking liquid culture media and TIS using RITA bioreactor. In vitro grown plantlets were screened for possible vegetative changes using agro-morphological descriptors while genetic and methylation differences were assessed using amplified fragment length polymorphism (AFLP) and methylation-sensitive amplification polymorphism (MSAP). In vitro results showed that the number of shoots produced in Musa spp. varied significantly (P≤0.001) with the type of culture system. The highest mean shoot produced was observed with TIS (28.40) and the least using semi-solid culture medium (1.13). For Dioscorea spp., there was no significant interaction between the hormone combination and the culture system. However, the lowest mean shoot value (1.55) was observed in the semi-solid culture medium. Genetic analysis via AFLP using 15 primer pair combinations revealed that the 3 culture systems maintained genetic variation for Musa and Dioscorea spp. under in vitro and field conditions. Results showed 99% and 91% of the total bands were polymorphic under in vitro and field conditions respectively for Musa and 100% polymorphism for Dioscorea under in vitro and field conditions. Methylation investigation via MSAP using 12 primer pair combinations showed 25% and 46% polymorphic methylated-sensitive loci, 100% and 78% of non-methylated loci of the total bands generated under in vitro and field conditions respectively. Unmethylated (HPA+/MSP+) levels were highest in TIS (0.0842) as compared to CI (0.0227) and SS (0.0161) while full methylation or absence of target (HPA-/MSP-) was lowest in TIS (0.5890) and highest in SS (0.7138). For Dioscorea, 52% and 53% methylated sensitive loci and 100% non-methylated loci were polymorphic under in vitro and field conditions respectively. Although in vitro plant tissue culture techniques led to methylation at some loci of both species, there were no observable changes in the phenotype of both crops under field conditions. This also confirmed that not all methylation events lead to phenotypic changes.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2019-05-16
dcterms.bibliographicCitationJekayinoluwa, T., Gueye, B., Bhattacharjee, R., Osibanjo, O., Shah, T. & Abberton, M. (2019). Agromorphologic, genetic and methylation profiling of Dioscorea and Musa species multiplied under three micropropagation systems. PLOS ONE, 14(5): e0216717, 1-17.en
dcterms.descriptionOpen Access Journalen
dcterms.extent1-17en
dcterms.issued2019-05-16
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherPublic Library of Scienceen
dcterms.subjectmusaen
dcterms.subjectdioscorea rotundataen
dcterms.subjectvitroplantsen
dcterms.subjecttissue cultureen
dcterms.subjectgeneticsen
dcterms.subjectmethylationen
dcterms.typeJournal Article

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