A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp.
| cg.authorship.types | CGIAR and developing country institute | en |
| cg.contributor.affiliation | Université de Dschang | en |
| cg.contributor.affiliation | University of Ibadan | en |
| cg.contributor.affiliation | International Institute of Tropical Agriculture | en |
| cg.coverage.country | Nigeria | en |
| cg.coverage.iso3166-alpha2 | NG | en |
| cg.coverage.region | Africa | en |
| cg.coverage.region | Western Africa | en |
| cg.howPublished | Formally Published | en |
| cg.issn | 0041-3291 | en |
| cg.issue | 2 | en |
| cg.journal | Tropical Science | en |
| cg.reviewStatus | Peer Review | en |
| cg.subject.iita | PLANT DISEASES | en |
| cg.subject.iita | YAM | en |
| cg.volume | 42 | en |
| dc.contributor.author | Njukeng, A.P. | en |
| dc.contributor.author | Ariri, G.I. | en |
| dc.contributor.author | Hughes, Jacqueline d'Arros | en |
| dc.contributor.author | Agindoran, B.O. | en |
| dc.contributor.author | Mignouna, Hodeba D. | en |
| dc.contributor.author | Thottappilly, G. | en |
| dc.date.accessioned | 2019-09-12T08:32:00Z | en |
| dc.date.available | 2019-09-12T08:32:00Z | en |
| dc.identifier.uri | https://hdl.handle.net/10568/103631 | |
| dc.title | A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp. | en |
| dcterms.abstract | A Nigerian isolate of Yam mosaic virus (YMV), genus Potyvirus from Dioscorea rotundata leaves was used to mechanically inoculate Nicotiana benthamiana (propagation host) plants from which the virus was purified. A hybridoma cell line secreting a monoclonal antibody (Mab) against YMV was produced by fusion of FOX-NY myeloma cells with immune splenocytes from in vivo immunizations of a Balb/C mouse. The polyclonal antiserum to YMV and the Mab (YMV-M24) were used in developing a triple antibody sandwich (TAS) enzyme-linked immunosorbent assay (ELISA) system for the detection of YMV from samples collected from fields. The test gave a positive reaction even at a sap dilution of 1:6250-1:31250 from leaf samples collected from different plants species, while antigen-coated plant (ACP) ELISA using the Mab could detect only to a dilution of 1:1250. TAS-ELISA was more sensitive and reliable when compared with ACP-ELISA using YMV-M24. YMV-M24 detected YMV isolates in some Dioscorea species from seven countries in the main yam growing area of West Africa. | en |
| dcterms.accessRights | Limited Access | en |
| dcterms.audience | Scientists | en |
| dcterms.bibliographicCitation | Njukeng, A.P., Atiri, G.I., D'A Hughes, J., Agindotan, B.O., Mignouna, H.D. & Thottappilly, G. (2002). A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp. Tropical Science, 42(2), 65-74. | en |
| dcterms.extent | 65-74 | en |
| dcterms.issued | 2002 | en |
| dcterms.language | en | en |
| dcterms.license | Copyrighted; all rights reserved | en |
| dcterms.subject | yams | en |
| dcterms.subject | plant viruses | en |
| dcterms.subject | monoclonal antibodies | en |
| dcterms.subject | dioscorea | en |
| dcterms.type | Journal Article | en |
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