Molecular genetic diversity and population structure of Ethiopian white lupin landraces: Implications for breeding and conservation

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationEthiopian Institute of Agricultural Researchen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationAddis Ababa Universityen
cg.contributor.affiliationInternational Maize and Wheat Improvement Centeren
cg.contributor.donorDepartment of Foreign Affairs and Trade, Australiaen
cg.contributor.donorSyngenta Foundation for Sustainable Agricultureen
cg.contributor.donorBill & Melinda Gates Foundationen
cg.contributor.donorDepartment for International Development, United Kingdomen
cg.contributor.donorSwedish International Development Cooperation Agencyen
cg.coverage.countryEthiopia
cg.coverage.iso3166-alpha2ET
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierMulugeta Atnaf Tiruneh: 0000-0002-3395-6303
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1371/journal.pone.0188696en
cg.isijournalISI Journalen
cg.issn1932-6203en
cg.issue11en
cg.journalPLOS ONEen
cg.reviewStatusPeer Reviewen
cg.subject.ilriANIMAL FEEDINGen
cg.subject.ilriBIODIVERSITYen
cg.subject.ilriBIOTECHNOLOGYen
cg.subject.ilriCROP-LIVESTOCKen
cg.subject.ilriCROPSen
cg.subject.ilriLEGUMESen
cg.volume12en
dc.contributor.authorTiruneh, Mulugeta Atnafen
dc.contributor.authorYao, N.en
dc.contributor.authorKyallo, Martina M.en
dc.contributor.authorDagne, K.en
dc.contributor.authorWegary, D.en
dc.contributor.authorTesfaye, K.en
dc.date.accessioned2017-12-13T08:46:53Zen
dc.date.available2017-12-13T08:46:53Zen
dc.identifier.urihttps://hdl.handle.net/10568/89713
dc.titleMolecular genetic diversity and population structure of Ethiopian white lupin landraces: Implications for breeding and conservationen
dcterms.abstractWhite lupin is one of the four economically important species of the Lupinus genus and is an important grain legume in the Ethiopian farming system. However, there has been limited research effort to characterize the Ethiopian white lupin landraces. Fifteen polymorphic simple sequence repeat (SSR) markers were used to assess the genetic diversity and population structure of 212 Ethiopian white lupin (Lupinus albus) landraces and two genotypes from different species (Lupinus angustifolius and Lupinus mutabilis) were used as out-group. The SSR markers revealed 108 different alleles, 98 of them from 212 landraces and 10 from out-group genotypes, with an average of 6.5 alleles per locus. The average gene diversity was 0.31. Twenty eight landraces harbored one or more private alleles from the total of 28 private alleles identified in the 212 white lupin accessions. Seventy-seven rare alleles with a frequency of less than 5% were identified and accounted for 78.6% of the total alleles detected. Analysis of molecular variance (AMOVA) showed that 92% of allelic diversity was attributed to individual accessions within populations while only 8% was distributed among populations. At 70% similarity level, the UPGMA dendrogram resulted in the formation of 13 clusters comprised of 2 to 136 landraces, with the out-group genotypes and five landraces remaining distinct and ungrouped. Population differentiation and genetic distance were relatively high between Gondar and Ethiopian white lupin populations collected by Australians. A model-based population structure analysis divided the white lupin landraces into two populations. All Ethiopian white lupin landrace populations, except most of the landraces collected by Australians (77%) and about 44% from Awi, were grouped together with significant admixtures. The study also suggested that 34 accessions, as core collections, were sufficient to retain 100% of SSR diversity. These accessions (core G-34) represent 16% of the whole 212 Ethiopian white lupin accessions and populations from West Gojam, Awi and Australian collections contributed more accessions to the core collection.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2017-11-30
dcterms.bibliographicCitationAtnaf, M., Yao, N., Kyalo, M., Dagne, K., Wegary, D. and Tesfaye, K. 2017. Molecular genetic diversity and population structure of Ethiopian white lupin landraces: Implications for breeding and conservation. PLoS ONE 12(11): e0188696.en
dcterms.issued2017-11-30
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherPublic Library of Scienceen
dcterms.subjectlegumesen
dcterms.subjectbiodiversityen
dcterms.subjectcropsen
dcterms.typeJournal Article

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