Equine Histoplasmosis in Ethiopia: Phylogenetic analysis by sequencing of the internal transcribed spacer region of rRNA genes

Simple item page
cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationAddis Ababa Universityen
cg.contributor.affiliationUnited Arab Emirates Universityen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.donorNational Human Genomic Instituteen
cg.coverage.countryEthiopiaen
cg.coverage.iso3166-alpha2ETen
cg.coverage.regionAfricaen
cg.coverage.regionEastern Africaen
cg.creator.identifierMartina kyalo: 0000-0001-9426-8152en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.3389/fcimb.2022.789157en
cg.isijournalISI Journalen
cg.issn2235-2988en
cg.journalFrontiers in Cellular and Infection Microbiologyen
cg.reviewStatusPeer Reviewen
cg.subject.ilriANIMAL DISEASESen
cg.volume12en
dc.contributor.authorAmeni, G.en
dc.contributor.authorMessele, A.en
dc.contributor.authorZewude, A.en
dc.contributor.authorGirma, M.en
dc.contributor.authorAsfa, R.en
dc.contributor.authorMamo, M.en
dc.contributor.authorKyallo, Martina M.en
dc.contributor.authorStomeo, Francescaen
dc.contributor.authorGumi, B.en
dc.contributor.authorSori, T.en
dc.date.accessioned2022-08-16T07:28:48Zen
dc.date.available2022-08-16T07:28:48Zen
dc.identifier.urihttps://hdl.handle.net/10568/120546
dc.titleEquine Histoplasmosis in Ethiopia: Phylogenetic analysis by sequencing of the internal transcribed spacer region of rRNA genesen
dcterms.abstractEquine histoplasmosis commonly known as epizootic lymphangitis (EL) is a neglected granulomatous disease of equine that is endemic to Ethiopia. It is caused by Histoplasma capsulatum variety farciminosum, a dimorphic fungus that is closely related to H. capsulatum variety capsulatum. The objective of this study was to undertake a phylogenetic analysis of H. capsulatum isolated from EL cases of horses in central Ethiopia and evaluate their relationship with H. capsulatum isolates in other countries and/or clades using the internal transcribed spacer (ITS) region of rRNA genes. Clinical and mycological examinations, DNA extraction, polymerase chain reaction (PCR), Sanger sequencing, and phylogenetic analysis were used for undertaking this study. Additionally, sequence data of Histoplasma isolates were retrieved from GenBank and included for a comprehensive phylogenetic analysis. A total of 390 horses were screened for EL and 97 were positive clinically while H. capsulatum was isolated from 60 horses and further confirmed with PCR, of which 54 were sequenced. BLAST analysis of these 54 isolates identified 29 H. capsulatum isolates and 14 isolates from other fungal genera while the remaining 11 samples were deemed insufficient for further downstream analysis. The phylogenetic analysis identified five clades, namely, African, Eurasian, North American 1 and 2, and Latin American A and B. The Ethiopian isolates were closely aggregated with isolates of the Latin American A and Eurasian clades, whereas being distantly related to isolates from North American 1 and 2 clades as well as Latin American B clade. This study highlights the possible origins and transmission routes of Histoplasmosis in Ethiopia.en
dcterms.accessRightsOpen Accessen
dcterms.audienceScientistsen
dcterms.available2022-07-08en
dcterms.bibliographicCitationAmeni, G., Messele, A., Zewude, A., Girma, M., Asfa, R., Mamo, M., Kyalo, M., Stomeo, F., Gumi, B. and Sori, T. 2022. Equine Histoplasmosis in Ethiopia: Phylogenetic analysis by sequencing of the internal transcribed spacer region of rRNA genes. Frontiers in Cellular and Infection Microbiology 12:789157.en
dcterms.issued2022-07-08en
dcterms.languageenen
dcterms.licenseCC-BY-4.0en
dcterms.publisherFrontiers Mediaen
dcterms.subjecthorsesen
dcterms.subjecthistoplasmosisen
dcterms.subjectanimal diseasesen
dcterms.typeJournal Articleen

Files

License bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ILRI articles in journals
ILRI BecA articles