Effect of an anti-methanogenic supplement on enteric methane emission, fermentation, and whole rumen metagenome in sheep

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationIndian Council of Agricultural Researchen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.donorIndian Council of Agricultural Researchen
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.3389/fmicb.2022.1048288en
cg.isijournalISI Journalen
cg.issn1664-302Xen
cg.journalFrontiers in Microbiologyen
cg.reviewStatusPeer Reviewen
cg.subject.ilriANIMAL FEEDINGen
cg.subject.ilriFEEDSen
cg.subject.ilriGHG EMISSIONSen
cg.subject.ilriSHEEPen
cg.subject.ilriSMALL RUMINANTSen
cg.volume13en
dc.contributor.authorMalik, P.K.en
dc.contributor.authorTrivedi, Shraddhaen
dc.contributor.authorKolte, A.P.en
dc.contributor.authorMohapatra, A.en
dc.contributor.authorBhatta, R.en
dc.contributor.authorRahman, Habibaren
dc.date.accessioned2022-12-14T10:14:20Zen
dc.date.available2022-12-14T10:14:20Zen
dc.identifier.urihttps://hdl.handle.net/10568/125959
dc.titleEffect of an anti-methanogenic supplement on enteric methane emission, fermentation, and whole rumen metagenome in sheepen
dcterms.abstractA study was conducted to investigate the impact of an anti-methanogenic product supplementation on enteric methane emissions, whole rumen metagenome and ruminal fermentation in sheep. Twelve adult male sheep were randomly divided into two groups of six animals each. Animals were fed ad libitum on a total mixed ration either without (CON) or with an anti-methanogenic supplement (Harit Dhara-HD). The anti-methanogenic supplement contained 22.1% tannic acid in a 3: 1 ratio of condensed and hydrolysable tannins. The supplementation of product revealed a significant reduction in daily enteric methane emission (21.9 vs. 17.2 g/d) and methane yield (23.2 vs. 18.2) without affecting the nutrient intake and digestibility. However, the propionate concentration in the HD treatment group was significantly higher than in the CON group. On the contrary, the ammonia nitrogen concentration was lower. The anti-methanogenic supplement significantly decreased the ruminal protozoa in the HD treatment group. Whole rumen metagenome analysis revealed that the core bacterial (Bacteroidetes and Firmicutes) and archaeal communities (Methanobrevibacter and Methanosarcina) were comparable between the CON and HD treatment groups. However, the supplementation of anti-methanogenic product led to a considerable reduction in the abundance of Proteobacteria, whereas the abundance of Lentisphaerae was greater. The supplementation significantly decreased the abundance of Methanocaldococcus, Methanococcoides, Methanocella, and Methanoregula methanogens. A total of 36 KO related to methanogenesis were identified in this study. The activities of formate dehydrogenase (EC 1.8.98.6) and tetrahydromethanopterin S-methyltransferase (EC 2.1.1.86) were significantly lowered by the anti-methanogenic product supplementation in sheep. In conclusion, the anti-methanogenic supplement has the potential to decrease enteric methane emission (~22%) at the recommended level (5% of DM) of supplementation. The contribution of minor methanogens vulnerable to supplementation to rumen methanogenesis is not known; hence, the culturing of these archaea should be taken on priority for determining the impact on overall rumen methanogenesis.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2022-11-21
dcterms.bibliographicCitationMalik, P.K., Trivedi, S., Kolte, A.P., Mohapatra, A., Bhatta, R. and Rahman, H. 2022. Effect of an anti-methanogenic supplement on enteric methane emission, fermentation, and whole rumen metagenome in sheep. Frontiers in Microbiology 13:1048288.en
dcterms.issued2022-11-21
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherFrontiers Mediaen
dcterms.subjectanimal feedingen
dcterms.subjectfeedsen
dcterms.subjectgreenhouse gas emissionsen
dcterms.subjectsmall ruminantsen
dcterms.subjectsheepen
dcterms.typeJournal Article

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