Prevalence and antibiotic resistance of Escherichia coli in urban and peri-urban garden ecosystems in Bangladesh

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cg.authorship.typesNot CGIAR developing country institute
cg.contributor.affiliationBangladesh Agricultural University
cg.contributor.affiliationBangabandhu Sheikh Mujibur Rahman Agricultural University
cg.contributor.affiliationCGIAR Initiative on Resilient Cities
cg.contributor.donorCGIAR Trust Fund
cg.contributor.initiativeResilient Cities
cg.howPublishedFormally Published
cg.identifier.doihttps://doi.org/10.1371/journal.pone.0315938
cg.isijournalISI Journal
cg.reviewStatusPeer Review
dc.contributor.authorPramanik, Pritom Kumar
dc.contributor.authorHoque, M. Nazmul
dc.contributor.authorRana, Md. Liton
dc.contributor.authorIslam, Md. Saiful
dc.contributor.authorUllah, Md. Ashek
dc.contributor.authorNeloy, Fahim Haque
dc.contributor.authorRamasamy, Srinivasan
dc.contributor.authorSchreinemachers, Pepijn
dc.contributor.authorOliva, Ricardo
dc.contributor.authorRahman, Md. Tanvir
dc.date.accessioned2025-02-11T15:54:18Z
dc.date.available2025-02-11T15:54:18Z
dc.identifier.urihttps://hdl.handle.net/10568/172958
dc.titlePrevalence and antibiotic resistance of Escherichia coli in urban and peri-urban garden ecosystems in Bangladesh
dcterms.abstractIn the past decade, there has been a notable rise in foodborne outbreaks, prominently featuring Escherichia coli as a primary pathogen. This bacterium, known for its prevalence in foodborne illnesses and as a reservoir of antimicrobial resistance, was isolated from raw vegetables, soil, and water samples collected from rooftop and surface gardens in urban (Dhaka North City Corporation; DNCC and Dhaka South City Corporation; DSCC) and periurban (Gazipur City Corporation; GCC) areas of Bangladesh. In this study, 145 samples including vegetables (n = 88), water (n = 27) and soils (n = 30) from DNCC (n = 85), DSCC (n = 30), and GCC (n = 30) were analyzed to assess the prevalence of E. coli using culture, biochemical tests, and PCR targeting the malB gene. E. coli was detected in 85 samples, indicating an overall prevalence of 58.62% (95% CI: 50.48–66.31). In urban areas (DNCC and DSCC), the prevalence rates were 44.70% and 80.0%, respectively, with surface gardens showing higher contamination rates (70.83%) than rooftop gardens (46.57%). In the peri-urban GCC, overall prevalence of E. coli was 76.7%, with rooftop gardens more contaminated (93.33%) than surface gardens (60.0%). Antibiogram profiling of 54 randomly selected isolates revealed 100% resistance to ampicillin, with varying resistance to ciprofloxacin (25.92%), tetracycline (14.81%), cotrimoxazole (14.81%), imipenem (9.25%), and fosfomycin (1.0%). Notably, all isolates were susceptible to ceftazidime, gentamicin, chloramphenicol, nitrofurantoin, and cefotaxime. Multidrug resistance (MDR) was found in 14.81% of isolates. The blaTEM gene was present in 81.48% of the isolates, while the tetA gene was detected in 3.70%. These findings underscore the urgent global health concern posed by the significant presence of E. coli in fresh vegetables, highlighting the need for improved safety measures and monitoring to prevent the spread of antimicrobial resistance through the food chain.
dcterms.accessRightsOpen Access
dcterms.audienceAcademics
dcterms.bibliographicCitationPramanik, P. K., Hoque, M. N., Rana, M. L., Islam, M. S., Ullah, M. A., Neloy, F. H., Ramasamy, S., Schreinemachers, P., Oliva, R. & Rahman, M. T. (2025). Prevalence and antibiotic resistance of Escherichia coli in urban and peri-urban garden ecosystems in Bangladesh. PLoS One 20(2): e0315938. https://doi.org/10.1371/journal.pone.0315938
dcterms.issued2025-02-06
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherPlos ONE
dcterms.subjectdomestic gardens
dcterms.subjectresistance to antibiotics
dcterms.typeJournal Article

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