A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta)

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cg.contributor.affiliationInternational Center for Tropical Agricultureen
cg.contributor.affiliationCentre de coopération internationale en recherche agronomique pour le développementen
cg.contributor.affiliationWageningen University and Researchen
cg.contributor.crpAgriculture for Nutrition and Health
cg.creator.identifierElise Talsma: 0000-0002-6034-4708
cg.creator.identifierAngélica M. Jaramillo: 0000-0002-7463-6816
cg.creator.identifierLuis Londoño: 0000-0003-0384-6055
cg.creator.identifierJohn Eiver Belalcazar Martinez: 0000-0002-4418-2526
cg.howPublishedFormally Publisheden
cg.identifier.dataurlhttps://doi.org/10.7910/dvn/d6gu8uen
cg.identifier.doihttps://doi.org/10.1371/journal.pone.0209702en
cg.isijournalISI Journalen
cg.issn1932-6203en
cg.issue12en
cg.journalPLOS ONEen
cg.numbere0209702en
cg.reviewStatusPeer Reviewen
cg.subject.ciatBIOFORTIFICATIONen
cg.subject.ciatCASSAVAen
cg.volume13en
dc.contributor.authorJaramillo Valencia, Angélica M.en
dc.contributor.authorLondoño Hernandez, Luis Fernandoen
dc.contributor.authorOrozco, Juan Camiloen
dc.contributor.authorPatiño, Gelveren
dc.contributor.authorBelalcázar, John Eiveren
dc.contributor.authorDavrieux, Fabriceen
dc.contributor.authorTalsma, Elise F.en
dc.date.accessioned2019-01-17T13:57:15Zen
dc.date.available2019-01-17T13:57:15Zen
dc.identifier.urihttps://hdl.handle.net/10568/99098
dc.titleA comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta)en
dcterms.abstractThe most commonly used method for measuring carotenoid concentration is high-performance liquid chromatography (HPLC). Nevertheless, easier, quicker, and less costly proxy methods exist. We aimed to determine the diagnostic performance of several proxy methods: the spectrophotometer, iCheck Carotene, and near-infrared spectroscopy using both a desktop (dNIRS) and a portable (pNIRS) device for the measurement of total carotenoid concentration (TCC) and all-trans-β-carotene concentration (trans-BC) in 30 fresh cassava (Manihot esculenta Crantz) storage roots in comparison with HPLC. The spectrophotometer presented the highest predictability for TCC, followed by iCheck, dNIRS, and pNIRS. The dNIRS showed the highest predictability and agreement for trans-BC. The pNIRS showed the poorest repeatability and greatest underestimations compared with HPLC. The agreement between all methods was lower for higher carotenoid concentration, with the exception of the spectrophotometer. According to our results, and for screening purposes, the measurement of carotenoids in fresh cassava roots can be carried out by spectrophotometer, iCheck Carotene and NIRS methods depending on the availability of equipment.en
dcterms.accessRightsOpen Access
dcterms.available2018-12-28
dcterms.bibliographicCitationJaramillo, Angélica M.; Londoño, Luis Fernando; Orozco, Juan Camilo; Patiño, Gelver; Belalcazar, John; Davrieux, Fabrice & Talsma, Elise F. (2018). A comparison study of five different methods to measure carotenoids in biofortified yellow cassava ( Manihot esculenta ). Plos One, 13(12): e0209702.en
dcterms.issued2018-12-28
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherPublic Library of Scienceen
dcterms.replaceshttps://hdl.handle.net/10568/171158en
dcterms.subjectbiofotificationen
dcterms.subjectmanihot esculentaen
dcterms.subjectcarotenoidsen
dcterms.subjectcassavaen
dcterms.subjectspectrophotometersen
dcterms.subjectequipmenten
dcterms.subjectboilingen
dcterms.subjectplant breedingen
dcterms.typeJournal Article

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