Report on sampling leaf tissue, DNA extraction and prepare for shipment for genotyping of two biparental populations to identify QTLs associated with Spittlebug resistance

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cg.authorship.typesCGIAR single centreen
cg.contributor.affiliationInternational Center for Tropical Agricultureen
cg.contributor.crpClimate Change, Agriculture and Food Security
cg.contributor.crpLivestock
cg.creator.identifierLuis M. Hernandez: 0000-0002-8816-0572
cg.creator.identifierDaniel M. Villegas: 0000-0001-6801-3332
cg.creator.identifierJacobo Arango: 0000-0002-4828-9398
cg.creator.identifierValheria Castiblanco: 0000-0003-2801-2153
cg.placeCali, Colombiaen
cg.subject.alliancebiovciatLIVESTOCKen
cg.subject.alliancebiovciatTROPICAL FORAGESen
cg.subject.impactAreaClimate adaptation and mitigation
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 2 - Zero hungeren
cg.subject.sdgSDG 12 - Responsible consumption and productionen
dc.contributor.authorEspitia, Paula A.en
dc.contributor.authorHernández, Luis Miguelen
dc.contributor.authorVillegas, Daniel Mauricioen
dc.contributor.authorArango, Jacoboen
dc.contributor.authorCastiblanco, Valheriaen
dc.date.accessioned2021-11-08T11:26:12Zen
dc.date.available2021-11-08T11:26:12Zen
dc.identifier.urihttps://hdl.handle.net/10568/115879
dc.titleReport on sampling leaf tissue, DNA extraction and prepare for shipment for genotyping of two biparental populations to identify QTLs associated with Spittlebug resistanceen
dcterms.abstractSpittlebugs (Hemiptera: Cercopidae) are the major production limitation for Brachiaria grasses (Urochloa spp.) in the tropical America. Incorporate host-plant resistance to new hybrids is the most cost-effective and sustainable solution to overcome the great losses caused by this key pest. Identifying regions in Urochloa genome associated with resistance to Aeneolamia varia nymphal attack, would allow to develop genotyping tools (e.g., molecular markers) that contribute to the hybrid’s selection in Urochloa interspecific breeding pipeline. Leaf tissue was extracted from 315 genotypes from the three biparental populations constructed for QTL analysis. These samples are kept at -20ºC, to be lyophilized and shipped for genotyping through RAD sequencing.en
dcterms.accessRightsOpen Access
dcterms.audienceCGIARen
dcterms.audienceDonorsen
dcterms.bibliographicCitationEspitia, P.; Hernández L.M.; Villegas, D.; Arango, J.; Castiblanco, V. (2021) Report on sampling leaf tissue, DNA extraction and prepare for shipment for genotyping of two biparental populations to identify QTLs associated with Spittlebug resistance. Cali (Colombia): Alliance of Bioversity and CIAT. 5 p.en
dcterms.extent5 p.en
dcterms.issued2021-09
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherAlliance of Bioversity International and CIATen
dcterms.subjectforageen
dcterms.subjectsamplingen
dcterms.subjectgenotypesen
dcterms.subjectdnaen
dcterms.subjectpest resistanceen
dcterms.subjectforrajesen
dcterms.subjectmuestreoen
dcterms.subjectgenotiposen
dcterms.subjectresistencia a las plagasen
dcterms.subjectadnen
dcterms.subjectcercopidaeen
dcterms.typeReport

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