An Ad/MVA vectored Theileria parva antigen induces schizont-specific CD8+ central memory T cells and confers partial protection against a lethal challenge

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cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationUniversity of Oxforden_US
cg.contributor.crpLivestocken_US
cg.contributor.donorBill & Melinda Gates Foundationen_US
cg.contributor.donorDepartment for International Development, United Kingdomen_US
cg.contributor.donorNorman Borlaug Commemorative Research Initiativeen_US
cg.contributor.donorUnited States Agency for International Developmenten_US
cg.contributor.donorUnited States Department of Agricultureen_US
cg.coverage.regionAfricaen_US
cg.coverage.regionEastern Africaen_US
cg.creator.identifierNicholas Svitek: 0000-0002-4539-5806en_US
cg.creator.identifierElizabeth Jane Poole: 0000-0002-8570-794Xen_US
cg.creator.identifierRoger Pelle: 0000-0003-1053-085Xen_US
cg.creator.identifierVishvanath Nene: 0000-0001-7066-4169en_US
cg.creator.identifierLucilla Steinaa: 0000-0003-3691-3971en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.1038/s41541-018-0073-5en_US
cg.isijournalISI Journalen_US
cg.issn2059-0105en_US
cg.issue1en_US
cg.journalNPJ Vaccinesen_US
cg.reviewStatusPeer Reviewen_US
cg.speciesTheileria parvaen_US
cg.subject.ilriANIMAL DISEASESen_US
cg.subject.ilriCATTLEen_US
cg.subject.ilriDISEASE CONTROLen_US
cg.subject.ilriECFen_US
cg.subject.ilriLIVESTOCKen_US
cg.subject.ilriVACCINESen_US
cg.volume3en_US
dc.contributor.authorSvitek, Nicholasen_US
dc.contributor.authorSaya, Rosemary M.en_US
dc.contributor.authorAwino, Eliasen_US
dc.contributor.authorMunyao, S.en_US
dc.contributor.authorMuriuki, R.en_US
dc.contributor.authorNjoroge, Thomasen_US
dc.contributor.authorPelle, Rogeren_US
dc.contributor.authorNdiwa, Nicholas N.en_US
dc.contributor.authorPoole, Elizabeth J.en_US
dc.contributor.authorGilbert, S.en_US
dc.contributor.authorNene, Vishvanath M.en_US
dc.contributor.authorSteinaa, Lucillaen_US
dc.date.accessioned2019-01-09T13:33:27Zen_US
dc.date.available2019-01-09T13:33:27Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/99007en_US
dc.titleAn Ad/MVA vectored Theileria parva antigen induces schizont-specific CD8+ central memory T cells and confers partial protection against a lethal challengeen_US
dcterms.abstractThe parasiteTheileria parvais the causative agent of East Coast fever (ECF), one of the most serious cattle diseases in sub-Saharan Africa, and directly impacts smallholder farmers’ livelihoods. There is an efficient live-parasite vaccine, but issues with transmission of vaccine strains, need of a cold chain, and antibiotics limit its utilization. This has fostered research towards subunit vaccination. Cytotoxic T lymphocytes (CTL) are crucial in combating the infection by lysingT. parva-infected cells. Tp1 is an immunodominant CTL antigen, which induces Tp1-specific responses in 70–80% of cattle of the A18 or A18v haplotype during vaccination with the live vaccine. In this study, human adenovirus serotype 5 (HAd5) and modified vaccinia Ankara (MVA) were assessed for their ability to induce Tp1-specific immunity. Both viral vectors expressing the Tp1 antigen were inoculated in cattle by a heterologous prime-boost vaccination regimen. All 15 animals responded to Tp1 as determined by ELISpot. Of these, 14 reacted to the known Tp1 epitope, assayed by ELISpot and tetramer analyses, with CTL peaking 1-week post-MVA boost. Eleven animals developed CTL with specific cytotoxic activity towards peripheral blood mononuclear cells (PBMC) pulsed with the Tp1 epitope. Moreover, 36% of the animals with a Tp1 epitope-specific response survived a lethal challenge withT. parva5 weeks post-MVA boost. Reduction of the parasitemia correlated with increased percentages of central memory lymphocytes in the Tp1 epitope-specific CD8+populations. These results indicate that Tp1 is a promising antigen to include in a subunit vaccine and central memory cells are crucial for clearing the parasite.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceScientistsen_US
dcterms.available2018-09-11en_US
dcterms.bibliographicCitationSvitek, N., Saya, R., Awino, E., Munyao, S., Muriuki, R., Njoroge, T., Pellé, R., Ndiwa, N., Poole, J., Gilbert, S., Nene, V. and Steinaa, L. 2018. An Ad/MVA vectored Theileria parva antigen induces schizont-specific CD8+ central memory T cells and confers partial protection against a lethal challenge. NPJ Vaccines 3:35.en_US
dcterms.issued2018-09-11en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.publisherSpringeren_US
dcterms.subjecttheileriaen_US
dcterms.subjectcattleen_US
dcterms.subjectanimal diseasesen_US
dcterms.subjectvaccinesen_US
dcterms.subjectdisease controlen_US
dcterms.subjecteast coast feveren_US
dcterms.typeJournal Articleen_US

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Improved vaccines for the control of East Coast fever in cattle in Africa