Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus

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cg.contributor.affiliationCapital Medical Universityen_US
cg.contributor.affiliationFondazione Edmund Machen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationJohn Innes Centreen_US
cg.contributor.affiliationNational Institute of Agricultural Botany, United Kingdomen_US
cg.contributor.affiliationQueensland University of Technologyen_US
cg.contributor.affiliationUniversity of Nottinghamen_US
cg.contributor.affiliationWhitehead Institute for Biomedical Researchen_US
cg.contributor.affiliationMassachusetts Institute of Technologyen_US
cg.contributor.affiliationGlobal Crop Diversity Trusten_US
cg.contributor.affiliationInternational Center for Agricultural Research in the Dry Areasen_US
cg.contributor.affiliationInstitute of Plant Molecular Biology, Czech Republicen_US
cg.contributor.affiliationUniversity of East Angliaen_US
cg.contributor.donorBiotechnology and Biological Sciences Research Council, United Kingdomen_US
cg.contributor.donorDeutsche Forschungsgemeinschaften_US
cg.contributor.donorFederal Ministry of Education and Research, Germanyen_US
cg.contributor.donorMinistry of Education Youth and Sportsen_US
cg.contributor.donorTempleton World Charity Foundationen_US
cg.contributor.donorCGIAR Trust Funden_US
cg.contributor.initiativeAccelerated Breedingen_US
cg.creator.identifierIsaac Njaci: 0000-0001-9649-6650en_US
cg.identifier.doihttps://doi.org/10.1038/s41467-023-36503-2en_US
cg.isijournalISI Journalen_US
cg.issn2041-1723en_US
cg.issue1en_US
cg.journalNature Communicationsen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.actionAreaGenetic Innovationen_US
cg.subject.ilriCROPSen_US
cg.subject.impactAreaNutrition, health and food securityen_US
cg.volume14en_US
dc.contributor.authorEdwards, Anneen_US
dc.contributor.authorNjaci, Isaacen_US
dc.contributor.authorSarkar, Abhimanyuen_US
dc.contributor.authorJiang, Zhouqianen_US
dc.contributor.authorKaithakottil, Gemy Georgeen_US
dc.contributor.authorMoore, Christopheren_US
dc.contributor.authorCheema, Jitenderen_US
dc.contributor.authorStevenson, Clare E.M.en_US
dc.contributor.authorRejzek, Martinen_US
dc.contributor.authorNovák, Petren_US
dc.contributor.authorVigouroux, Marielleen_US
dc.contributor.authorVickers, Martinen_US
dc.contributor.authorWouters, Roland H.M.en_US
dc.contributor.authorPaajanen, Piritaen_US
dc.contributor.authorSteuernagel, Burkharden_US
dc.contributor.authorMoore, Jonathan D.en_US
dc.contributor.authorHiggins, Janeten_US
dc.contributor.authorSwarbreck, Daviden_US
dc.contributor.authorMartens, Stefanen_US
dc.contributor.authorKim, Colin Y.en_US
dc.contributor.authorWeng, Jing-Keen_US
dc.contributor.authorMundree, Sagadevanen_US
dc.contributor.authorKilian, Benjaminen_US
dc.contributor.authorKumar, Shiven_US
dc.contributor.authorLoose, Matten_US
dc.contributor.authorYant, Levien_US
dc.contributor.authorMacas, Jiříen_US
dc.contributor.authorWang, Trevor L.en_US
dc.contributor.authorMartin, Cathieen_US
dc.contributor.authorEmmrich, Peter M.F.en_US
dc.date.accessioned2023-03-10T14:38:58Zen_US
dc.date.available2023-03-10T14:38:58Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/129553en_US
dc.titleGenomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativusen_US
dcterms.abstractGrass pea ( Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a changing climate. The lack of genetic resources and the crop’s association with the disease neurolathyrism have limited the cultivation of grass pea. Here, we present an annotated, long read-based assembly of the 6.5 Gbp L. sativus genome. Using this genome sequence, we have elucidated the biosynthetic pathway leading to the formation of the neurotoxin, β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP). The final reaction of the pathway depends on an interaction between L. sativus acyl-activating enzyme 3 (LsAAE3) and a BAHD-acyltransferase (LsBOS) that form a metabolon activated by CoA to produce β-L-ODAP. This provides valuable insight into the best approaches for developing varieties which produce substantially less toxin.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.available2023-02-16en_US
dcterms.bibliographicCitationEdwards, Anne; Njaci, Isaac; Sarkar, Abhimanyu; Jiang, Zhouqian; Kaithakottil, Gemy George; Moore, Christopher; Cheema, Jitender; Stevenson, Clare E.M.; Rejzek, Martin; Novák, Petr; Vigouroux, Marielle; Vickers, Martin; Wouters, Roland H.M.; Paajanen, Pirita; Steuernagel, Burkhard; Moore, Jonathan D.; Higgins, Janet; Swarbreck, David; Martens, Stefan; Kim, Colin Y.; Weng, Jing-Ke; Mundree, Sagadevan; Kilian, Benjamin; Kumar, Shiv; Loose, Matt; Yant, Levi; Macas, Jiří; Wang, Trevor L.; Martin, Cathie; Emmrich, Peter M.F. 2023. Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus. Nature Communications 14:en_US
dcterms.issued2023-02-16en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.publisherSpringeren_US
dcterms.subjectgenomicsen_US
dcterms.subjectbiosynthesisen_US
dcterms.subjectlathyrusen_US
dcterms.typeJournal Articleen_US

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