Development of a potential yeast-based vaccine platform for Theileria parva infection in cattle

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cg.authorship.typesNot CGIAR international instituteen_US
cg.contributor.affiliationRoyal Veterinary College, United Kingdomen_US
cg.contributor.affiliationKing's College Londonen_US
cg.contributor.affiliationMoredun Research Instituteen_US
cg.contributor.crpLivestocken_US
cg.contributor.donorDepartment for International Development, United Kingdomen_US
cg.contributor.donorBill & Melinda Gates Foundationen_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.3389/fimmu.2021.674484en_US
cg.isijournalISI Journalen_US
cg.issn1664-3224en_US
cg.journalFrontiers in Immunologyen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.ilriANIMAL DISEASESen_US
cg.subject.ilriCATTLEen_US
cg.subject.ilriDISEASE CONTROLen_US
cg.subject.ilriECFen_US
cg.subject.ilriLIVESTOCKen_US
cg.subject.ilriVACCINESen_US
cg.volume12en_US
dc.contributor.authorGoh, S.en_US
dc.contributor.authorKolakowski, J.en_US
dc.contributor.authorHolder, A.en_US
dc.contributor.authorPfuhl, M.en_US
dc.contributor.authorNgugi, D.en_US
dc.contributor.authorBallingall, Keith T.en_US
dc.contributor.authorTombacz, K.en_US
dc.contributor.authorWerling, D.en_US
dc.date.accessioned2021-07-14T09:23:12Zen_US
dc.date.available2021-07-14T09:23:12Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/114269en_US
dc.titleDevelopment of a potential yeast-based vaccine platform for Theileria parva infection in cattleen_US
dcterms.abstractEast Coast Fever (ECF), caused by the tick-borne apicomplexan parasiteTheileria parva, remains one of the most important livestock diseases in sub-Saharan Africa with more than 1 million cattle dying from infection every year. Disease prevention relies on the so-called “Infection and Treatment Method” (ITM), which is costly, complex, laborious, difficult to standardise on a commercial scale and results in a parasite strain-specific, MHC class I-restricted cytotoxic T cell response. We therefore attempted to develop a safe, affordable, stable, orally applicable and potent subunit vaccine for ECF using five differentT. parvaschizont antigens (Tp1, Tp2, Tp9, Tp10 and N36) andSaccharomyces cerevisiaeas an expression platform. Full-length Tp2 and Tp9 as well as fragments of Tp1 were successfully expressed on the surface ofS. cerevisiae.In vitroanalyses highlighted that recombinant yeast expressing Tp2 can elicit IFNγ responses using PBMCs from ITM-immunized calves, while Tp2 and Tp9 induced IFNγ responses from enriched bovine CD8+T cells. A subsequentin vivostudy showed that oral administration of heat-inactivated, freeze-dried yeast stably expressing Tp2 increased total murine serum IgG over time, but more importantly, induced Tp2-specific serum IgG antibodies in individual mice compared to the control group. While these results will require subsequent experiments to verify induction of protection in neonatal calves, our data indicates that oral application of yeast expressing Theileria antigens could provide an affordable and easy vaccination platform for sub-Saharan Africa. Evaluation of antigen-specific cellular immune responses, especially cytotoxic CD8+T cell immunity in cattle will further contribute to the development of a yeast-based vaccine for ECF.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceScientistsen_US
dcterms.available2021-07-08en_US
dcterms.bibliographicCitationGoh, S., Kolakowski, J., Holder, A., Pfuhl, M., Ngugi, D., Ballingall, K., Tombacz, K. and Werling, D. 2021. Development of a potential yeast-based vaccine platform for Theileria parva infection in cattle. Frontiers in Immunology 12:674484.en_US
dcterms.issued2021-07-08en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.publisherFrontiers Mediaen_US
dcterms.subjecttheileria parvaen_US
dcterms.subjectanimal diseasesen_US
dcterms.subjecteast coast feveren_US
dcterms.subjectdisease controlen_US
dcterms.subjectcattleen_US
dcterms.subjectlivestocken_US
dcterms.subjectvaccinesen_US
dcterms.typeJournal Articleen_US

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Improved vaccines for the control of East Coast fever in cattle in Africa
CRP Livestock health flagship