An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems

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cg.authorship.typesCGIAR and developing country instituteen_US
cg.contributor.affiliationJomo Kenyatta University of Agriculture and Technologyen_US
cg.contributor.affiliationInternational Institute of Tropical Agricultureen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.donorSwedish International Development Cooperation Agencyen_US
cg.contributor.donorDepartment of Foreign Affairs and Trade, Australiaen_US
cg.contributor.donorSyngenta Foundation for Sustainable Agricultureen_US
cg.contributor.donorBill & Melinda Gates Foundationen_US
cg.contributor.donorDepartment for International Development, United Kingdomen_US
cg.coverage.countryKenyaen_US
cg.coverage.iso3166-alpha2KEen_US
cg.coverage.regionAfricaen_US
cg.coverage.regionEastern Africaen_US
cg.creator.identifierSita Ghimire: 0000-0001-8930-1384en_US
cg.creator.identifierIsaac Njaci: 0000-0001-9649-6650en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.5897/ajb2020.17115en_US
cg.issn1684-5315en_US
cg.issue7en_US
cg.journalAfrican Journal of Biotechnologyen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.ilriAGRICULTUREen_US
cg.subject.ilriBIOTECHNOLOGYen_US
cg.subject.ilriCROPSen_US
cg.subject.ilriRESEARCHen_US
cg.volume20en_US
dc.contributor.authorKepue, M.S.en_US
dc.contributor.authorMweu, C.M.en_US
dc.contributor.authorKariuki, D.M.en_US
dc.contributor.authorKerubo, L.en_US
dc.contributor.authorNjaci, Isaacen_US
dc.contributor.authorNjoroge, Anneen_US
dc.contributor.authorKago, Leahen_US
dc.contributor.authorMware, B.O.en_US
dc.contributor.authorGhimire, Sita R.en_US
dc.date.accessioned2021-07-29T14:29:14Zen_US
dc.date.available2021-07-29T14:29:14Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/114461en_US
dc.titleAn efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristemsen_US
dcterms.abstractTaro (Colocasia esculenta L. Schott) is an important crop for food and nutrition security, incomes, and livelihood of people in developing countries. Its cultivation and productivity have been limited mainly due to a lack of quality planting materials. Therefore, this study developed an effective micropropagation method for two common taro varieties in Kenya (Purple Wild and Dasheen) using apical meristem derived from portions of the corm and base of leaf petioles. This study optimized the 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA) levels for a better shoot and root development. The highest shoot induction was observed in both varieties when Murashige & Skoog (MS) media was supplemented with 2 mg/L of BAP. Similarly, the maximum rooting response was achieved in both varieties on half-strength MS media supplemented with 0.5 mg/L of IBA. The study also revealed significant interactions between variety × BAP levels and variety × IBA levels (P<0.0001) for shoot and root development, respectively. This apical meristem-based micropropagation method developed in this study can be used for rapid multiplication of genetically clean planting materials for commercial uses and to establish taro transformation protocols.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceScientistsen_US
dcterms.available2021-07-31en_US
dcterms.bibliographicCitationKepue, M.S., Mweu, C.M., Kariuki, D.M., Kerubo, L., Njaci, I., Njoroge, A., Kago, L., Mware, B.O. and Ghimire, S.R. 2021. An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems. African Journal of Biotechnology 20(7):293-299.en_US
dcterms.extentp. 293-299en_US
dcterms.issued2021-07-31en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.publisherAcademic Journalsen_US
dcterms.subjectcrop improvementen_US
dcterms.subjectlivelihoodsen_US
dcterms.subjectcropsen_US
dcterms.subjectbiotechnologyen_US
dcterms.typeJournal Articleen_US

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