An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationJomo Kenyatta University of Agriculture and Technologyen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.donorSwedish International Development Cooperation Agencyen
cg.contributor.donorDepartment of Foreign Affairs and Trade, Australiaen
cg.contributor.donorSyngenta Foundation for Sustainable Agricultureen
cg.contributor.donorBill & Melinda Gates Foundationen
cg.contributor.donorDepartment for International Development, United Kingdomen
cg.coverage.countryKenya
cg.coverage.iso3166-alpha2KE
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierSita Ghimire: 0000-0001-8930-1384
cg.creator.identifierIsaac Njaci: 0000-0001-9649-6650
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.5897/ajb2020.17115en
cg.issn1684-5315en
cg.issue7en
cg.journalAfrican Journal of Biotechnologyen
cg.reviewStatusPeer Reviewen
cg.subject.ilriAGRICULTUREen
cg.subject.ilriBIOTECHNOLOGYen
cg.subject.ilriCROPSen
cg.subject.ilriRESEARCHen
cg.volume20en
dc.contributor.authorKepue, M.S.en
dc.contributor.authorMweu, C.M.en
dc.contributor.authorKariuki, D.M.en
dc.contributor.authorKerubo, L.en
dc.contributor.authorNjaci, Isaacen
dc.contributor.authorNjoroge, Anneen
dc.contributor.authorKago, Leahen
dc.contributor.authorMware, B.O.en
dc.contributor.authorGhimire, Sita R.en
dc.date.accessioned2021-07-29T14:29:14Zen
dc.date.available2021-07-29T14:29:14Zen
dc.identifier.urihttps://hdl.handle.net/10568/114461
dc.titleAn efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristemsen
dcterms.abstractTaro (Colocasia esculenta L. Schott) is an important crop for food and nutrition security, incomes, and livelihood of people in developing countries. Its cultivation and productivity have been limited mainly due to a lack of quality planting materials. Therefore, this study developed an effective micropropagation method for two common taro varieties in Kenya (Purple Wild and Dasheen) using apical meristem derived from portions of the corm and base of leaf petioles. This study optimized the 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA) levels for a better shoot and root development. The highest shoot induction was observed in both varieties when Murashige & Skoog (MS) media was supplemented with 2 mg/L of BAP. Similarly, the maximum rooting response was achieved in both varieties on half-strength MS media supplemented with 0.5 mg/L of IBA. The study also revealed significant interactions between variety × BAP levels and variety × IBA levels (P<0.0001) for shoot and root development, respectively. This apical meristem-based micropropagation method developed in this study can be used for rapid multiplication of genetically clean planting materials for commercial uses and to establish taro transformation protocols.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2021-07-31
dcterms.bibliographicCitationKepue, M.S., Mweu, C.M., Kariuki, D.M., Kerubo, L., Njaci, I., Njoroge, A., Kago, L., Mware, B.O. and Ghimire, S.R. 2021. An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems. African Journal of Biotechnology 20(7):293-299.en
dcterms.extentp. 293-299en
dcterms.issued2021-07-31
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherAcademic Journalsen
dcterms.subjectcrop improvementen
dcterms.subjectlivelihoodsen
dcterms.subjectcropsen
dcterms.subjectbiotechnologyen
dcterms.typeJournal Article

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