Cassava haplotype map highlights fixation of deleterious mutations during clonal propagation
| cg.authorship.types | CGIAR and developing country institute | en |
| cg.contributor.affiliation | Cornell University | en |
| cg.contributor.affiliation | National Crops Resources Research Institute, Uganda | en |
| cg.contributor.affiliation | International Institute of Tropical Agriculture | en |
| cg.contributor.affiliation | National Root Crops Research Institute, Nigeria | en |
| cg.contributor.affiliation | University of California | en |
| cg.contributor.affiliation | Donald Danforth Plant Science Center | en |
| cg.contributor.affiliation | United States Department of Agriculture | en |
| cg.contributor.crp | Roots, Tubers and Bananas | |
| cg.contributor.donor | Bill & Melinda Gates Foundation | en |
| cg.howPublished | Formally Published | en |
| cg.identifier.doi | https://doi.org/10.1038/ng.3845 | en |
| cg.identifier.iitatheme | BIOTECH & PLANT BREEDING | |
| cg.isijournal | ISI Journal | en |
| cg.issn | 1061-4036 | en |
| cg.issue | 6 | en |
| cg.journal | Nature Genetics | en |
| cg.reviewStatus | Peer Review | en |
| cg.subject.iita | CASSAVA | en |
| cg.subject.iita | PLANT BREEDING | en |
| cg.volume | 49 | en |
| dc.contributor.author | Ramu, P. | en |
| dc.contributor.author | Esuma, W. | en |
| dc.contributor.author | Kawuki, R.S. | en |
| dc.contributor.author | Rabbi, Ismail Y. | en |
| dc.contributor.author | Egesi, Chiedozie N. | en |
| dc.contributor.author | Bredeson, J.V. | en |
| dc.contributor.author | Bart, R.S. | en |
| dc.contributor.author | Verma, J. | en |
| dc.contributor.author | Buckler, E.S. | en |
| dc.contributor.author | Lu, F | en |
| dc.date.accessioned | 2017-05-15T09:35:21Z | en |
| dc.date.available | 2017-05-15T09:35:21Z | en |
| dc.identifier.uri | https://hdl.handle.net/10568/81024 | |
| dc.title | Cassava haplotype map highlights fixation of deleterious mutations during clonal propagation | en |
| dcterms.abstract | Cassava (Manihot esculenta Crantz) is an important staple food crop in Africa and South America; however, ubiquitous deleterious mutations may severely decrease its fitness. To evaluate these deleterious mutations, we constructed a cassava haplotype map through deep sequencing 241 diverse accessions and identified >28 million segregating variants. We found that (i) although domestication has modified starch and ketone metabolism pathways to allow for human consumption, the concomitant bottleneck and clonal propagation have resulted in a large proportion of fixed deleterious amino acid changes, increased the number of deleterious alleles by 26%, and shifted the mutational burden toward common variants; (ii) deleterious mutations have been ineffectively purged, owing to limited recombination in the cassava genome; (iii) recent breeding efforts have maintained yield by masking the most damaging recessive mutations in the heterozygous state but have been unable to purge the mutation burden; such purging should be a key target in future cassava breeding. | en |
| dcterms.accessRights | Open Access | |
| dcterms.audience | Scientists | en |
| dcterms.available | 2017-04-17 | |
| dcterms.bibliographicCitation | Ramu, P., Esuma, W., Kawuki, R., Rabbi, I.Y., Egesi, C., Bredeson, J.V., ... & Lu, F. (2017). Cassava haplotype map highlights fixation of deleterious mutations during clonal propagation. Nature Genetics. | en |
| dcterms.extent | p. 959-963 | en |
| dcterms.issued | 2017-06 | |
| dcterms.language | en | |
| dcterms.license | CC-BY-4.0 | |
| dcterms.publisher | Springer | en |
| dcterms.subject | genomics | en |
| dcterms.subject | plant genetics | en |
| dcterms.subject | population genetics | en |
| dcterms.subject | cassava | en |
| dcterms.subject | deleterious mutations | en |
| dcterms.subject | haplotype map | en |
| dcterms.subject | clonal propagation | en |
| dcterms.type | Journal Article |