Factors influencing Agrobacterium-mediated genetic transformation efficiency in bread wheat (Triticum aestivum L.)

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cg.contributor.affiliationBorlaug Institute for South Asiaen
cg.contributor.affiliationInternational Maize and Wheat Improvement Centeren
cg.contributor.affiliationFaculty of Agriculture, SGT Universityen
cg.contributor.donorCGIAR Trust Funden
cg.contributor.initiativeAccelerated Breeding
cg.creator.identifierKarthikeyan Thiyagarajan: 0000-0003-2864-9373
cg.creator.identifierGovindan Velu: 0000-0001-9502-4352
cg.creator.identifierPrashant Vikram: 0000-0002-3740-6608
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.31742/isgpb.84.1.5en
cg.isijournalISI Journalen
cg.issn0019-5200en
cg.issue1en
cg.journalIndian Journal of Genetics and Plant Breedingen
cg.reviewStatusPeer Reviewen
cg.subject.actionAreaGenetic Innovation
cg.subject.impactAreaNutrition, health and food security
cg.volume84en
dc.contributor.authorThiyagarajan, Karthikeyanen
dc.contributor.authorNoguera, Luis Miguelen
dc.contributor.authorPacheco, Marioen
dc.contributor.authorGovindan, Veluen
dc.contributor.authorVikram, Prashanten
dc.date.accessioned2024-11-22T15:34:08Zen
dc.date.available2024-11-22T15:34:08Zen
dc.identifier.urihttps://hdl.handle.net/10568/162630
dc.titleFactors influencing Agrobacterium-mediated genetic transformation efficiency in bread wheat (Triticum aestivum L.)en
dcterms.abstractAssessment of the efficiency of Agrobacterium tumefaciens mediated transformation in four bread wheat varieties was focused and upon optimization of experiments done to obtain tangible results. The four varieties, Fielder, Navojoa, Baj and Kachu/saul, were transformed with optimized protocols and randomly selected from a selection medium amalgamated with phosphinothricin (Glufosinate). Initially, the variety Fielder has proceeded with 36 different concentrations of synthetic auxins 2,4-D and picloram as 36 treatments. The treatment 30 (48 µg of 2,4-D and 64 µg of picloram) has shown higher efficiency and is comparable in terms of the three traits studied. Additionally, antioxidants and growth regulators were adjusted to obtain better results towards reasonable transformation frequency. Additional varieties were also tested and selected against the selectable marker PPT based on the optimization for the variety Fielder. Selected genotypes were subjected for gusA or Bar gene PCR amplification. The result revealed the frequency of transgenesis in the range of 60 to 70% with the varieties studied. However, the whole experiment needs further repetitions for confirmatory results. Transgenic genotypes of the Fielder variety showed moderate to strong GUS expression in leaves, anthers, seeds, seed coats, and roots compared to non-transgenic control plant tissues. PPT leaf painting assay showed the lack of necrosis on the painted area of leaves in transgenic genotypes compared to the non-transgenic ones.en
dcterms.accessRightsOpen Access
dcterms.available2024-04-10
dcterms.bibliographicCitationThiyagarajan, K., Noguera, L. M., Pacheco, M., Govindan, V., & Vikram, P. (2024). Factors influencing Agrobacterium-mediated genetic transformation efficiency in bread wheat (Triticum aestivum L.). Indian Journal Of Genetics And Plant Breeding (The), 84(01), 63-72. https://doi.org/10.31742/isgpb.84.1.5en
dcterms.extentp. 63-72en
dcterms.hasVersionhttps://hdl.handle.net/10883/35043en
dcterms.issued2024-04-10
dcterms.languageen
dcterms.licenseCC-BY-NC-ND-4.0
dcterms.publisherIndian Society of Genetics and Plant Breedingen
dcterms.subjectantioxidantsen
dcterms.subjectassessmenten
dcterms.subjectsoft wheaten
dcterms.subjectexperimentationen
dcterms.typeJournal Article

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