Detection and Characterization of Seed-Borne Bacterial Leaf Blight in Wheat

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cg.contributor.affiliationInternational Center for Agricultural Research in the Dry Areasen_US
cg.contributor.affiliationGeneral Commission for Scientific Agricultural Researchen_US
cg.contributor.affiliationUniversity of Aleppo, Faculty of Agricultureen_US
cg.contributor.donorCGIAR Trust Funden_US
cg.contributor.initiativeGenebanksen_US
cg.coverage.countryLebanonen_US
cg.coverage.countrySyrian Arab Republicen_US
cg.coverage.iso3166-alpha2LBen_US
cg.coverage.iso3166-alpha2SYen_US
cg.coverage.regionWestern Asiaen_US
cg.creator.identifierSafaa Kumari: 0000-0002-4492-6257en_US
cg.creator.identifierAbdulrahman Moukahel: 0000-0003-4297-771Xen_US
cg.identifier.urlhttps://www.icpp2023.org/sites/default/files/2023-08/ICPP2023-BOOK-ABSTRACTS-VF.pdfen_US
cg.subject.actionAreaGenetic Innovationen_US
cg.subject.impactAreaNutrition, health and food securityen_US
cg.subject.impactAreaPoverty reduction, livelihoods and jobsen_US
cg.subject.impactAreaGender equality, youth and social inclusionen_US
cg.subject.impactAreaEnvironmental health and biodiversityen_US
cg.subject.sdgSDG 1 - No povertyen_US
cg.subject.sdgSDG 2 - Zero hungeren_US
cg.subject.sdgSDG 15 - Life on landen_US
cg.subject.sdgSDG 17 - Partnerships for the goalsen_US
dc.contributor.authorKumari, Safaaen_US
dc.contributor.authorMoukahel, Abdulrahmanen_US
dc.contributor.authorAbo Bakr, Abdoen_US
dc.contributor.authorKassem, Mohammaden_US
dc.date.accessioned2023-12-11T20:26:25Zen_US
dc.date.available2023-12-11T20:26:25Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/135232en_US
dc.titleDetection and Characterization of Seed-Borne Bacterial Leaf Blight in Wheaten_US
dcterms.abstractWheat is one of the most important crops which contributes toward global food security and represents a main source of food and income for millions of smallholder farmers worldwide. Pseudomonas syringae pv. syringae (Pss) is the causal agent of bacterial leaf blight wheat disease which can cause up to 50% yield loss or more depending on the time of infection and region. In addition, Pss is transmitted by wheat seeds, which can play a role in longdistance spread. Therefore, developing and implementing effective management strategies for bacterial diseases is very important to reduce yield and quality loss. However, rapid and accurate detection of diseases is the first essential step for effective management strategies for control of this disease. Even though molecular tools for Pss precise detection and characterization has been developed, the most practical approach for rapid diagnosis is the use of serological assays using specific antibodies. A polyclonal antiserum against a Syrian isolate of Pss was produced and its quality was evaluated by Dot-blot Immunoassay using homologous and heterologous antigens. Results revealed that the produced antiserum was able to detect Pss up to 1×103 CFU/ml dilution using raw antiserum at a dilution of 1/160 with no cross reactivity with other bacterial species (e.g. Xanthomonas).en_US
dcterms.accessRightsOpen Accessen_US
dcterms.available2023-08-21en_US
dcterms.bibliographicCitationKumari, S, A. R. Moukahel, A. Abo Bakr and M. Kassem. 2023. Detection and characterization of seed-borne bacterial leaf blight in wheat. Page 347. In: 12th International Congress of Plant Pathology, 20-25 August 2023, Lyon, France.en_US
dcterms.formatPDFen_US
dcterms.issued2023-08-21en_US
dcterms.languageenen_US
dcterms.licenseCopyrighted; Non-commercial educational use onlyen_US
dcterms.publisherInternational Society for Plant Pathologyen_US
dcterms.subjectserological diagnosisen_US
dcterms.subjectbacterial blighten_US
dcterms.subjectpseudomonas syringae (p.s.)en_US
dcterms.subjectseed borne diseasesen_US
dcterms.typeConference Paperen_US

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