Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus

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cg.contributor.affiliationInternational Rice Research Instituteen
cg.contributor.affiliationUniversity of Minnesotaen
cg.identifier.doihttps://doi.org/10.1111/pbi.12927en
cg.issn1467-7644en
cg.issue11en
cg.journalPlant Biotechnology Journalen
cg.volume16en
dc.contributor.authorMacovei, Ancaen
dc.contributor.authorSevilla, Neah R.en
dc.contributor.authorCantos, Christianen
dc.contributor.authorJonson, Gilda B.en
dc.contributor.authorSlamet-Loedin, Inezen
dc.contributor.authorČermák, Tomášen
dc.contributor.authorVoytas, Daniel F.en
dc.contributor.authorChoi, Il-Ryongen
dc.contributor.authorChadha-Mohanty, Prabhjiten
dc.date.accessioned2024-12-19T12:54:26Zen
dc.date.available2024-12-19T12:54:26Zen
dc.identifier.urihttps://hdl.handle.net/10568/164882
dc.titleNovel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virusen
dcterms.abstractRice tungro disease (RTD) is a serious constraint in rice production across tropical Asia. RTD is caused by the interaction between Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus. RTSV resistance found in traditional cultivars has contributed to a reduction in the incidence of RTD in the field. Natural RTSV resistance is a recessive trait controlled by the translation initiation factor 4 gamma gene (eIF4G). The Y1059V1060V1061 residues of eIF4G are known to be associated with the reactions to RTSV. To develop new sources of resistance to RTD, mutations in eIF4G were generated using the CRISPR/Cas9 system in the RTSV‐susceptible variety IR64, widely grown across tropical Asia. The mutation rates ranged from 36.0% to 86.6%, depending on the target site, and the mutations were successfully transmitted to the next generations. Among various mutated eIF4G alleles examined, only those resulting in in‐frame mutations in SVLFPNLAGKS residues (mainly NL), adjacent to the YVV residues, conferred resistance. Furthermore, our data suggest that eIF4G is essential for normal development, as alleles resulting in truncated eIF4G could not be maintained in homozygous state. The final products with RTSV resistance and enhanced yield under glasshouse conditions were found to no longer contain the Cas9 sequence. Hence, the RTSV‐resistant plants with the novel eIF4G alleles represent a valuable material to develop more diverse RTSV‐resistant varieties.en
dcterms.accessRightsOpen Access
dcterms.available2018-04-30
dcterms.bibliographicCitationMacovei, Anca; Sevilla, Neah R.; Cantos, Christian; Jonson, Gilda B.; Slamet‐Loedin, Inez; Čermák, Tomáš; Voytas, Daniel F.; Choi, Il‐Ryong and Chadha‐Mohanty, Prabhjit. 2018. Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus. Plant Biotechnology Journal, Volume 16 no. 11 p. 1918-1927en
dcterms.extentpp. 1918-1927en
dcterms.issued2018-11
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherWileyen
dcterms.subjectallelesen
dcterms.subjectdisease resistanceen
dcterms.subjectmutationsen
dcterms.subjectplant virusesen
dcterms.subjectresistance genesen
dcterms.subjectrice tungro virusen
dcterms.typeJournal Article

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