Molecular characterization of a synthetic neutralizing antibody targeting p67 of Theileria parva

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cg.authorship.typesCGIAR and advanced research institute
cg.contributor.affiliationUniversity of Waterloo
cg.contributor.affiliationUniversity of Toronto
cg.contributor.affiliationAbtech Therapeutics, France
cg.contributor.affiliationInternational Livestock Research Institute
cg.contributor.donorInternational Development Research Centre
cg.contributor.donorCanada Foundation for Innovation
cg.contributor.donorGates Foundation
cg.creator.identifierAnna Lacasta: 0000-0001-8381-9844
cg.creator.identifierHannah Chege: 0009-0005-1984-7855
cg.creator.identifierVishvanath Nene: 0000-0001-7066-4169
cg.howPublishedFormally Published
cg.identifier.doihttps://doi.org/10.1002/pro.70153
cg.isijournalISI Journal
cg.issn0961-8368
cg.issue6
cg.journalProtein Science
cg.reviewStatusPeer Review
cg.speciesTheileria parva
cg.subject.ilriDISEASE CONTROL
cg.subject.ilriECF
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 2 - Zero hunger
cg.volume34
dc.contributor.authorMiersch, S.
dc.contributor.authorSinger, A.U.
dc.contributor.authorChen, C.
dc.contributor.authorFellouse, F.
dc.contributor.authorGopalsamy, A.
dc.contributor.authorCosta, L.S. e
dc.contributor.authorLacasta, Anna
dc.contributor.authorChege, Hannah
dc.contributor.authorChege, Naomi
dc.contributor.authorNene, Vishvanath
dc.contributor.authorSidhu, S.S.
dc.date.accessioned2025-05-19T13:59:21Z
dc.date.available2025-05-19T13:59:21Z
dc.identifier.urihttps://hdl.handle.net/10568/174653
dc.titleMolecular characterization of a synthetic neutralizing antibody targeting p67 of Theileria parva
dcterms.abstractThe <i>Theileria parva</i> sporozoite surface antigen p67 is a target of the bovine humoral immune response that generates antibodies capable of providing protection against subsequent infection. As a result, p67 has been the subject of efforts aimed at the development of an anti-sporozoite subunit vaccine. Previous studies have identified neutralizing epitopes in the N- and C-terminal regions of the full-length protein and shown that immunization with a C-terminal fragment of p67 (p67C) alone is capable of eliciting protection. To identify additional neutralizing epitopes in p67C, selections were conducted against it using a phage-displayed synthetic antibody library. An antibody that neutralized the sporozoite in vitro was identified, and the crystal structure of a Fab:peptide complex was elucidated. Mutagenesis studies aimed at validating and further characterizing the Fab:peptide interaction identified critical residues involved in binding and neutralization. This study also validates distinct epitopes for previously reported neutralizing antibodies.
dcterms.accessRightsOpen Access
dcterms.audienceAcademics
dcterms.audienceScientists
dcterms.available2025-05-19
dcterms.bibliographicCitationMiersch, S., Singer, A.U., Chen, C., Fellouse, F., Gopalsamy, A., Costa, L.S. e, Lacasta, A., Chege, H., Chege, N., Nene, V. and Sidhu, S.S. 2025. Molecular characterization of a synthetic neutralizing antibody targeting p67 of <i>Theileria parva</i>. Protein Science 34(6): e70153.
dcterms.extente70153
dcterms.issued2025-06
dcterms.languageen
dcterms.licenseCC-BY-NC-4.0
dcterms.publisherWiley
dcterms.subjectdisease control
dcterms.subjecteast coast fever
dcterms.typeJournal Article

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