Detection and Partial Characterization of Polerovirus and Luteovirus Isolates Associated With Lentil and Chickpea in Ethiopia

Simple item page
cg.contributor.affiliationInternational Center for Agricultural Research in the Dry Areasen
cg.contributor.affiliationAddis Ababa Universityen
cg.contributor.affiliationAmhara National Regional State, Amhara Bureau of Agricultureen
cg.contributor.affiliationAmhara Regional Agricultural Research Institute, Gondar Agricultural Research Centeren
cg.contributor.affiliationBotswana International University of Science & Technologyen
cg.contributor.donorCGIAR Trust Funden
cg.contributor.initiativePlant Healthen
cg.coverage.countryEthiopiaen
cg.coverage.iso3166-alpha2ETen
cg.coverage.regionEastern Africaen
cg.creator.identifierSafaa Kumari: 0000-0002-4492-6257en
cg.creator.identifierAbdulrahman Moukahel: 0000-0003-4297-771Xen
cg.creator.identifierSeid Kemal: 0000-0002-1791-9369en
cg.identifier.doihttps://doi.org/10.1002/leg3.70024en
cg.isijournalISI Journalen
cg.issn2639-6181en
cg.issue1en
cg.journalLegume Scienceen
cg.reviewStatusPeer Reviewen
cg.subject.actionAreaResilient Agrifood Systemsen
cg.subject.impactAreaClimate adaptation and mitigationen
cg.subject.impactAreaNutrition, health and food securityen
cg.subject.impactAreaPoverty reduction, livelihoods and jobsen
cg.subject.impactAreaEnvironmental health and biodiversityen
cg.subject.sdgSDG 1 - No povertyen
cg.subject.sdgSDG 2 - Zero hungeren
cg.subject.sdgSDG 15 - Life on landen
cg.subject.sdgSDG 17 - Partnerships for the goalsen
cg.volume7en
dc.contributor.authorAdeme, Antenehen
dc.contributor.authorKumari, Safaaen
dc.contributor.authorMoukahel, Abdulrahmanen
dc.contributor.authorAlemu, Tesfayeen
dc.contributor.authorAbraham, Adaneen
dc.contributor.authorAynewa, Yetsedawen
dc.contributor.authorGuadie, Demsachewen
dc.contributor.authorKemal, Seid Ahmeden
dc.date.accessioned2025-02-20T17:43:38Zen
dc.date.available2025-02-20T17:43:38Zen
dc.identifier.urihttps://hdl.handle.net/10568/173298
dc.titleDetection and Partial Characterization of Polerovirus and Luteovirus Isolates Associated With Lentil and Chickpea in Ethiopiaen
dcterms.abstractThe partial nucleotide sequence of the coat protein (CP) gene of Ethiopian isolates of chickpea chlorotic stunt virus (CpCSV, genus Polerovirus), beet western yellows virus (BWYV, genus Polerovirus), and soybean dwarf virus (SbDV, genus Luteovirus) was determined from lentil and chickpea plants showing yellowing, stunting, and reddening symptoms. Comparative sequence analysis of CpCSV isolates obtained from five chickpea and five lentil isolates showed 94.9%–100% and 91.9%–98.7% nucleotide sequence identity with each other and with the reference isolates, respectively. One CpCSV isolate from chickpea (MZ043728) showed a close relationship with isolates of the serotype II while the remaining nine isolates were closely related to isolates belonging to serotype I. Sequence identities of three chickpea BWYV isolates varied from 93.3% to 100% with the reference isolates, and one of them (MZ043727) displayed 100% nucleotide identity with previously reported lentil stunt virus (LStV, genus Polerovirus). The chickpea isolates MZ043725 and MZ043726 appear to be identical to each other, whereas the other isolate (MZ043727) was identical to previously identified LStV isolate. The nucleotide sequence of three Ethiopian SbDV isolates had a lower identity with GenBank isolates and their phylogenetic analysis showed that they are clustered separately from the rest of the reference isolates indicating that they are the most divergent. This result generates essential information for further research on legume viruses in Ethiopia. In addition, a detailed study should be conducted in the future to understand the prevalence of LStV and determine the potential yield losses associated with the virus in Ethiopia.en
dcterms.accessRightsOpen Accessen
dcterms.available2025-02-05en
dcterms.bibliographicCitationAdeme, A. Kumari, S. G. Moukahel, A. Alemu, T. Abraham, A. Aynewa, Y. Guadie, D. and Ahmed, S. 2025. Detection and Partial Characterization of Polerovirus and Luteovirus Isolates Associated With Lentil and Chickpea in Ethiopia. Legume Science, 7(1): e70024. https://doi.org/10.1002/leg3.70024en
dcterms.formatPDFen
dcterms.issued2025-02-05en
dcterms.languageenen
dcterms.licenseCC-BY-4.0en
dcterms.publisherWiley Open Accessen
dcterms.subjectphylogenetic analysisen
dcterms.subjectlentilen
dcterms.subjectchickpeaen
dcterms.subjectsequencingen
dcterms.subjectsbdven
dcterms.subjectcpcsven
dcterms.subjectbwyven
dcterms.typeJournal Articleen

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
43ca23f018800b46f4ad2726b996de2e.pdf
Size:
953.87 KB
Format:
Adobe Portable Document Format
Description:
Journal Article
Download

License bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

MEL submissions
CGIAR Initiative on Plant Health
ICARDA Articles in Journals
ICARDA Publications