Maximizing laboratory production of aflatoxins and fumonisins for use in experimental animal feeds

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.authorship.typesConsultanten
cg.contributor.affiliationUniversity of Liègeen
cg.contributor.affiliationGhent Universityen
cg.contributor.affiliationChuka Universityen
cg.contributor.affiliationUniversity of Nairobien
cg.contributor.affiliationDSM - BIOMIN Research Centeren
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationUppsala Universityen
cg.contributor.affiliationSwedish University of Agricultural Sciencesen
cg.contributor.donorMinistry of Education, Science and Technology, Kenyaen
cg.contributor.donorFonds de la Recherche Scientifique, Belgiumen
cg.contributor.donorBelgian Science Policy Officeen
cg.coverage.countryKenya
cg.coverage.iso3166-alpha2KE
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierJohanna Lindahl: 0000-0002-1175-0398
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.3390/microorganisms10122385en
cg.isijournalISI Journalen
cg.issn2076-2607en
cg.issue12en
cg.journalMicroorganismsen
cg.reviewStatusPeer Reviewen
cg.subject.ilriAFLATOXINSen
cg.subject.ilriFEEDSen
cg.subject.ilriFOOD SAFETYen
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 2 - Zero hungeren
cg.volume10en
dc.contributor.authorOchieng, P.E.en
dc.contributor.authorKemboi, D.C.en
dc.contributor.authorScippo, M.L.en
dc.contributor.authorGathumbi, J.K.en
dc.contributor.authorKang'ethe, Erastus K.en
dc.contributor.authorDoupovec, B.en
dc.contributor.authorCroubels, S.en
dc.contributor.authorLindahl, Johanna F.en
dc.contributor.authorAntonissen, G.en
dc.contributor.authorOkoth, S.en
dc.date.accessioned2022-12-27T07:06:58Zen
dc.date.available2022-12-27T07:06:58Zen
dc.identifier.urihttps://hdl.handle.net/10568/126311
dc.titleMaximizing laboratory production of aflatoxins and fumonisins for use in experimental animal feedsen
dcterms.abstractWarm and humid climatic conditions coupled with poor agricultural practices in sub-Saharan Africa favor the contamination of food and feed by Aspergillus flavus and Fusarium verticillioides fungi, which subsequently may produce aflatoxins (AFs) and fumonisins (FBs), respectively. The growth of fungi and the production of mycotoxins are influenced by physical (temperature, pH, water activity, light and aeration), nutritional, and biological factors. This study aimed at optimizing the conditions for the laboratory production of large quantities of AFs and FBs for use in the animal experiments. A. flavus and F. verticillioides strains, previously isolated from maize in Kenya, were used. Levels of AFB1 and total FBs (FB1, FB2, and FB3) in different growth substrates were screened using ELISA methods. Maize kernels inoculated with three different strains of A. flavus simultaneously and incubated at 29 °C for 21 days had the highest AFB1 level of 12,550 ± 3397 μg/kg of substrate. The highest level of total FBs (386,533 ± 153,302 μg/kg of substrate) was detected in cracked maize inoculated with three different strains of F. verticillioides and incubated for 21 days at temperatures of 22–25 °C in a growth chamber fitted with yellow light. These two methods are recommended for the mass production of AFB1 and FBs for animal feeding trials.en
dcterms.accessRightsOpen Access
dcterms.audienceAcademicsen
dcterms.audienceScientistsen
dcterms.available2022-11-30
dcterms.bibliographicCitationOchieng, P.E., Kemboi, D.C., Scippo, M.L., Gathumbi, J.K., Kang'ethe, E., Doupovec, B., Croubels, S., Lindahl, J.F., Antonissen, G. and Okoth, S. 2022. Maximizing laboratory production of aflatoxins and fumonisins for use in experimental animal feeds. Microorganisms 10(12): 2385.en
dcterms.extent2385en
dcterms.issued2022-11-30
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherMDPIen
dcterms.subjectaflatoxinsen
dcterms.subjectfumonisinsen
dcterms.subjectfeedsen
dcterms.subjectfood safetyen
dcterms.typeJournal Article

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