Chromosome painting facilitates anchoring reference genome sequence to chromosomes In Situ and integrated karyotyping in banana (Musa Spp.)

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cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationCzech Academy of Sciencesen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.affiliationBioversity Internationalen
cg.contributor.affiliationKatholieke Universiteit Leuvenen
cg.contributor.crpRoots, Tubers and Bananas
cg.creator.identifierBrigitte Uwimana: 0000-0001-7460-9001
cg.creator.identifierRony Swennen: 0000-0002-5258-9043
cg.creator.identifierJaroslav Dolezel: 0000-0002-6263-0492
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.3389/fpls.2019.01503en
cg.identifier.iitathemeBIOTECH & PLANT BREEDING
cg.isijournalISI Journalen
cg.issn1664-462Xen
cg.journalFrontiers in Plant Scienceen
cg.reviewStatusPeer Reviewen
cg.subject.iitaAGRONOMYen
cg.subject.iitaBANANAen
cg.subject.iitaPLANT BREEDINGen
cg.subject.iitaPLANT GENETIC RESOURCESen
cg.subject.iitaPLANT HEALTHen
cg.subject.iitaPLANT PRODUCTIONen
cg.volume10en
dc.contributor.authorŠimoníková, D.en
dc.contributor.authorNěmečková, A.en
dc.contributor.authorKarafiátová, M.en
dc.contributor.authorUwimana, Brigitteen
dc.contributor.authorSwennen, Rony L.en
dc.contributor.authorDoležel, Jaroslaven
dc.contributor.authorHřibová, E.en
dc.date.accessioned2019-12-19T09:41:18Zen
dc.date.available2019-12-19T09:41:18Zen
dc.identifier.urihttps://hdl.handle.net/10568/106239
dc.titleChromosome painting facilitates anchoring reference genome sequence to chromosomes In Situ and integrated karyotyping in banana (Musa Spp.)en
dcterms.abstractOligo painting FISH was established to identify all chromosomes in banana (Musa spp.) and to anchor pseudomolecules of reference genome sequence of Musa acuminata spp. malaccensis “DH Pahang” to individual chromosomes in situ. A total of 19 chromosome/chromosome-arm specific oligo painting probes were developed and were shown to be suitable for molecular cytogenetic studies in genus Musa. For the first time, molecular karyotypes of diploid M. acuminata spp. malaccensis (A genome), M. balbisiana (B genome), and M. schizocarpa (S genome) from the Eumusa section of Musa, which contributed to the evolution of edible banana cultivars, were established. This was achieved after a combined use of oligo painting probes and a set of previously developed banana cytogenetic markers. The density of oligo painting probes was sufficient to study chromosomal rearrangements on mitotic as well as on meiotic pachytene chromosomes. This advance will enable comparative FISH mapping and identification of chromosomal translocations which accompanied genome evolution and speciation in the family Musaceae.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2019-11-20
dcterms.bibliographicCitationŠimoníková, D., Němečková, A., Karafiátová, M., Uwimana, B., Swennen, R., Doležel, J. & Hřibová, E. (2019). Chromosome painting facilitates anchoring reference genome sequence to chromosomes in situ and integrated karyotyping in banana (Musa spp.). Frontiers in Plant Science, 10, 1-12.en
dcterms.extent1-12en
dcterms.issued2019
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherFrontiers Mediaen
dcterms.subjectbananasen
dcterms.subjectchromosomesen
dcterms.subjectkaryotypesen
dcterms.subjectmusaen
dcterms.subjectdnaen
dcterms.subjectgenomesen
dcterms.typeJournal Article

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