Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.)

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cg.creator.identifierStephen E Beebe: 0000-0002-3742-9930
cg.creator.identifierJoe Tohme: 0000-0003-2765-7101
cg.identifier.urlhttp://www.plantsciences.ucdavis.edu/GEPTS/Blair%20et%20al.%202003.pdfen
cg.isijournalISI Journalen
cg.issn1432-2242en
cg.issue8en
cg.journalTheoretical and Applied Geneticsen
cg.reviewStatusPeer Reviewen
cg.subject.ciatBEANSen
cg.subject.ciatBIODIVERSITYen
cg.subject.ciatGENETIC RESOURCESen
cg.volume107en
dc.contributor.authorBlair, Matthew W.en
dc.contributor.authorPedraza García, Fabioen
dc.contributor.authorBuendía Castellanos, Hector F.en
dc.contributor.authorGaitán Solís, Elianaen
dc.contributor.authorBeebe, Stephen E.en
dc.contributor.authorGepts, Paul L.en
dc.contributor.authorTohme, Joseph M.en
dc.date.accessioned2014-09-24T08:41:58Zen
dc.date.available2014-09-24T08:41:58Zen
dc.identifier.urihttps://hdl.handle.net/10568/43330
dc.titleDevelopment of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.)en
dcterms.abstractA total of 150 microsatellite markers developed for common bean (Phaseolus vulgaris L.) were tested for parental polymorphism and used to determine the positions of 100 genetic loci on an integrated genetic map of the species. The value of these single-copy markers was evident in their ability to link two existing RFLP-based genetic maps with a base map developed for the Mesoamerican × Andean population, DOR364 × G19833. Two types of microsatellites were mapped, based respectively on gene-coding and anonymous genomic-sequences. Gene-based microsatellites proved to be less polymorphic (46.3%) than anonymous genomic microsatellites (64.3%) between the parents of two inter-genepool crosses. The majority of the microsatellites produced single bands and detected single loci, however four of the gene-based and three of the genomic microsatellites produced consistent double or multiple banding patterns and detected more than one locus. Microsatellite loci were found on each of the 11 chromosomes of common bean, the number per chromosome ranging from 5 to 17 with an average of ten microsatellites each. Total map length for the base map was 1,720 cM and the average chromosome length was 156.4 cM, with an average distance between microsatellite loci of 19.5 cM. The development of new microsatellites from sequences in the Genbank database and the implication of these results for genetic mapping, quantitative trait locus analysis and marker-assisted selection in common bean are described.en
dcterms.accessRightsOpen Access
dcterms.bibliographicCitationBlair, Matthew W.; Pedraza García, Fabio; Buendía Castellanos, Héctor Fabio; Gaitán Solís, Eliana; Beebe, Stephen E.; Gepts, Paul L.; Tohme M., Joseph. 2003. Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.) . Theoretical and Applied Genetics (Germany) 107(8):1362-1374.en
dcterms.extentp. 1362-1374en
dcterms.issued2003
dcterms.languageen
dcterms.subjectphaseolus vulgarisen
dcterms.subjectgenetic markersen
dcterms.subjectmicrosatellitesen
dcterms.subjectgenetic mapsen
dcterms.subjectgenetic polymorphismen
dcterms.subjectmarcadores genéticosen
dcterms.subjectmicrosatélitesen
dcterms.subjectmapas genéticosen
dcterms.subjectpolimorfismo genéticoen
dcterms.typeJournal Article

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