Complete nucleotide sequences of the infectious cloned DNAs of bean dwarf mosaic geminivirus

Bean dwarf mosaic geminivirus (BDMV) was shown to have a bipartite genome (DNA-A and DNA-B) from restriction map and sequence data comparisons of cloned DNAs. The nucleotide sequences of the infectious clones of DNA-A and DNA-B were determined. Computer analyses showed that the bipartite genome of BDMV resembles that of other whitefly-transmitted geminiviruses. The DNA-A (2,615 nt) and DNA-B (2,575 nt) have little sequence homology other than that within the common region. The nucleotide sequences of the 187-nt common regions of the two DNAs had 96% identity and included a region of 33 nt that can form a stem-loop structure nearly identical to that of other geminiviruses. Nucleotide sequence analysis identified six open reading frames, four on DNA-A and two on DNA-B, involving viral and complementary strand sense transcription. Comparisons of nucleotide and derived amino acid sequences of BDMV with those of other whitefly-transmitted geminiviruses showed that BDMV was most closely related to Abutilon mosaic geminivirus and not closely related to bean golden mosaic geminivirus. BDMV can be distinguished from other bean-infecting geminiviruses by differences in symptoms, host range, reaction of bean germplasm, and sequence identities; thus, BDMV is a distinct bean-infecting geminivirus.