Characterization of Zebu cattle breeds in Tanzania using random amplified polymorphic DNA markers

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cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationSokoine University of Agricultureen
cg.coverage.countryTanzania
cg.coverage.iso3166-alpha2TZ
cg.coverage.regionEastern Africa
cg.creator.identifierStephen J Kemp: 0000-0003-4041-1720
cg.identifier.doihttps://doi.org/10.1111/j.1365-2052.1994.tb00085.xen
cg.issn0268-9146en
cg.issn1365-2052en
cg.issue2en
cg.journalAnimal Geneticsen
cg.subject.ilriCATTLEen
cg.subject.ilriGENETICSen
cg.volume25en
dc.contributor.authorGwakisa, P.S.en
dc.contributor.authorKemp, Stephen J.en
dc.contributor.authorTeale, A.J.en
dc.date.accessioned2023-03-10T14:39:59Zen
dc.date.available2023-03-10T14:39:59Zen
dc.identifier.urihttps://hdl.handle.net/10568/129577
dc.titleCharacterization of Zebu cattle breeds in Tanzania using random amplified polymorphic DNA markersen
dcterms.abstractA total of 141 short primers, of arbitrary nucleotide sequence, were used singly in poly‐merase chain reactions to amplify DNA fingerprints in pools of DNA representing three Zebu cattle breeds. Two primers, which discriminated between the breed‐specific DNA pools were used further to amplify individual pool components in order to establish band frequencies of the amplified fingerprints. One of the primers (ILO 1127) amplified a RAPD fingerprint in 61%of TSZ animals but less than 6% in the other breeds, while another primer (ILO 1065) revealed a DNA sequence common to 89% of the Boran animals and less than 30% in the other two breeds. Bandsharing and mean average percentage difference calculated within and between the three breeds using RAPD fingerprint data showed a higher degree of homogeneity within than across the breeds and indicated measurable divergence between the three breeds. It is concluded that RAPD polymorphisms are useful as genetic markers for cattle breed differentiation.en
dcterms.accessRightsLimited Access
dcterms.available2009-04-24
dcterms.bibliographicCitationGwakisa, P S; Kemp, Stephen J.; Teale, A.J. 2009. Characterization of Zebu cattle breeds in Tanzania using random amplified polymorphic DNA markers. Animal Genetics 25: 89-94en
dcterms.extentp. 89-94en
dcterms.issued2009-04-24
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherWileyen
dcterms.subjectcattleen
dcterms.subjectbreedsen
dcterms.subjectdnaen
dcterms.subjectzebu cattleen
dcterms.subjecttanzaniaen
dcterms.subjectcattle breedsen
dcterms.subjectzebuen
dcterms.subjectrandom amplified polymorphic dnaen
dcterms.typeJournal Article

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