CAAS-ILRI Joint Laboratory outputs
Permanent URI for this collectionhttps://hdl.handle.net/10568/2422
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Item Joint laboratory on livestock and forage genetic resources(Brochure, 2013-10-31) International Livestock Research InstituteItem Polymorphism of DSG4 gene and Its association with wool traits in fur sheep(Journal Article, 2011-05-15) Zhang, Ge; Zhang, Yuan-yue; Han Jianlin; Guan, Wei-Jun; Li, Ying-kang; Xu, Bin; Zhang, Xiu-tao; Ma, Yue-huiItem Genetic diversity of an experimental population of Jianbai Xiang pig(Journal Article, 2009) Gong, Wei-hua; Yang, Shu-lin; Cui, Wei-tao; Gan, Shi-xiang; Li, Kui; Tang, Zhong-lin; Han JianlinItem Study on single nucleotide polymorphisms of H -FABP gene in sheep(Journal Article, 2009-08-14) Qiao, H.Y.; Zhao, Q.J.; Yao, N.; Lu, Y.-Z.; Meng, X.-R.; Guan, Wei-Jun; Ma, Y.H.; Han JianlinPCR-SSCP and DNA sequencing approaches were applied to assess the single nucleotide polymorphisms (SNPs) and analyze the genetic polymorphisms at partial exon 2 and intron 2 of H-FABP(Heart fatty acid-binding protein)gene, and five sheep populations that comprised of Small-Tailed Han sheep (SH, 48), Ningxia Tan sheep (Tan, 121), Tan × SH F1 (23), Poll Dorset (48) and Suffolk (24) sheep were screened in this stuty. The result showed: (1)four SNPs at 981(G/A), 1014(A/C) 1019(T/C) and 1058 (-/G) and nine genotypes (AA, BB, CC, AB, AC, BC, AD, CD and BD) were detected using primer 2, the AA was the predominant genotype. A chi-square analysis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium except the Tan and Suffolk populations. Statistical analysis revealed a low polymorphism information content (PIC) in the Suffolk and Tan × SH F1 populations (PIC< 0.25) but an intermediate PIC in the remaining three populations (0.25< PIC <0.50). It meant that the fragment of H-FABP had polymorphisms, which could be used as a candidate gene associated gene with phenotypic traits like intramuscular fat content in different sheep populations. (2)Three genotypes (HH, Hh and hh) determined by a SNP at 2407(T-C) were detected using primer 4. The genotype frequencies were in the order of HH＞Hh＞hh. A chi-square analy-sis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium in the Tan and Poll Dorset populations, and the PIC values were low (PIC < 0.25). However, there was no polymorphisms in SH, Tan × SH F1 and Suffolk populations.Item Identification of SNP at nucleotide position 1892 of coding sequence of the chicken Mx gene(Journal Article, 2009) Jin Lina; Luo Yuzhu; Han JianlinItem Assessment of genetic relationships among the Plateau Merino populations in China using microsatellite DNA markers(Journal Article, 2009) Yu-Zhu Luo; Shu-ru Cheng; Hu, J.; Hanotte, Olivier H.; Han JianlinAssessment of genetic relationships among the Chinese Plateau Merino breeds was made by using microsatellite DNA markers for the purposes of understanding of their genetic background and providing basic information for supporting conservation of genetic resources and genetic improvement. 【Method】 Using 15 microsatellite DNA markers, the Gansu Alpine Merino and the Qinghai Merino populations were analyzed along with the other two foreign sheep breeds and seven indigenous Chinese sheep populations in order to examine their population genetic structure and differentiation. 【Result】 The phylogenetic analysis, principal component analysis and the Bayesian clustering approach were employed to analyse the data and it was observed that the 11 populations conld be divided into three genetic types. In particular, the Gansu Alpine Merino and Qinghai Merino formed a single cluster. 【Conclusion】 This study, using microsatellite DNA makers, reveals that the Gansu Alpine Merino and Qinghai Merino have a similar genetic background, in alignment with their breeding histories, thus they can be treated as a single genetic unit in future genetic resource conservation and improvement programs.Item Genetic diversity in the mtDNA D-Loop region of wild yak (Bos grunniens mutus)(Journal Article, 2009-11-20) Ma Zhi-Iie; Zhong Jin-Cheng; Xu Jing-Tao; Dou Quan-Lin; Chang Huai-Pu; Han JianlinItem Genetic diversity and phylogenetic relationship of Chinese cashmere goats based on microsatellite DNA markers(Journal Article, 2007) Di, R.; He, X.; Guan, Wei-Jun; Pu, Y.; Zhao, Qianjun; Fu, B.; Ma, Y.; Han JianlinGenetic diversity of nine indigenous Chinese cashmere goat populations and one West African breed were investigated using 19 microsatellite DNA markers and fluorescence PCR. The aim was to investigate the status of the genetic resources of Chinese cashmere goats. Fourteen of the microsatellite loci were highly polymorphic and effective markers for analysis of genetic diversity and relationship among goat populations. Analysis of polymorphic information content and genetic heterozygosity showed that the Chinese goat populations have high genetic diversity. Both UPGMA dendrogram and scatter plots of estimates from principal component analyses showed that the Chinese and African goat populations belong to different clades. Among the Chinese goat populations the Hegu and Hexi populations were genetically more distant from the other goat populations. The remaining seven Chinese goat populations were clustered into two clades. The first clade contained Liaoning, Xinjiang, Chaidamu and Shanbei goat populations, while the sec-ond clade was made up of goat populations from Inner Mongolia. These results may provide a scientific basis for the characteristization, conservation and utilization of Chinese cashmere goats.Item Sheep genetic diversity in Bhutan using microsatellite markers(Journal Article, 2010-04) Tashi, D.; Wafula, P.; Yoshio, Y.; Shinji, S.; Kenji, O.; Hanotte, Olivier H.; Bang-Zhong, L.; Hideyuki, M.; Han JianlinGenotype data from eight microsatellite markers were used to assess genetic diversity and relationships among five indigenous Bhutanese sheep populations, Sakten, Jakar, Sarpang, Sipsu and Tsirang. Estimates of mean observed and expected heterozygosities, mean number of alleles per locus/population were obtained. The highest observed heterozygosities were found in Jakar (0.657) and Sakten (0.647), while the lowest one was found in Tsirang (0.539). Genetic distances, pairwise proportion of different alleles, UPGMA tree, and principal component analysis indicate close relationship among Tsirang, Sipsu and Sarpang populations, while Jakar and Sakten populations are located in one cluster. These two clusters are separated geographically, and show distinct phenotypic as well as molecular characters. We therefore recommend that the Bhutanese native sheep populations be classified into at least two distinct breeds, Jakar-Sakten sheep and Sipsu sheep. Since Jakar and Sakten sheep have different morphological phenotypes, further analyses will be required to understand the genetic differences between these two sheep populations.