IITA SOP
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Standard Operating Procedures
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Item Standard Operating Procedures for harvesting and harvest evaluation in cassava(Training Material, 2024-06-10) Akpotuzor, P.; Iluebbey, P.; Ayankanmi, T.; Oke, E.; Olaniyo, E.; Ogunpaimo, K.; Andrew, S.In cassava breeding, harvesting is carried out to determine the root yield and assess other important traits. It marks the end of the fieldwork for an experimental cassava trial. Cassava field trials should be ready for harvesting 9-12 months after planting. Harvesting is done manually by a team composed of supervisors, technicians, and daily rated staff.Item Standard Operating Procedure for high throughput phenotyping using NIRS(Training Material, 2024-06-10) Mbanjo-Edwige, G.; Peteti, P.; Toyinbo, S.; Ogunpaimo, K.; Nafiu, K.The near-infrared spectroscopy is a non-destructive and rapid method for predicting the qualitative and quantitative properties of samples. The NIRS spectral regions, which are located between the visible and infrared ranges (800-2500 nm; 12500-4000 cm-1) contain a wealth of useful information. The development and optimization of NIRS prediction models could enable reliable, high-throughput, and low-cost analyses of high-value traits. Hundreds of samples could be quickly screened, and multiple parameters could be evaluated in a single test.Item Standard Operating Procedure (SOP) for leaf sample collection for genotyping(Training Material, 2024-06-10) Olutegbe, A.; Nnadi, A.; Ige, A.; Nafiu, K.; Ogungbesan, B.; Kayondo, S.I.; Mbanjo-Edwige, G.; Rabbi, I.Application of deoxyribonucleic acid (DNA) technology in plant breeding offers an opportunity to enhance and accelerate genetic gain. Molecular markers, which are DNA or gene sequences with known locations on chromosomes are used as a tool for identification and genetic analysis. DNA extraction is the starting point and a critical step for genetic analysis. DNA can be extracted from diverse sources, including seeds and leaves of plants. Proper plant collection and storage conditions are essential for successfully extracting DNA of high quality and quantity from plants.Item Standard Operating Procedure (SOP): trial management(Training Material, 2024-06-10) Ayankanmi, T.; Parkes, E.; Rabbi, I.; Kulakow, P.; Iluebbey, P.; Ekanem, U.; Akpotuzor, P.; Olaniyo, E.; Oke, E.; Ogunpaimo, K.Field trials are important in cassava breeding research. Cassava breeders conduct a variety of field trials in the process of developing improved varieties. The results of these trials are used to take informed decisions regarding the variety to release. Good field management can help ensure consistency and repeatability of field trials. Therefore, proper management of field trials is critical for making accurate decisions.Item Standard Operating Procedure (SOP): weed control and management(Training Material, 2024-06-10) Ayankanmi, T.; Iluebbey, P.; Kulakow, P.; Parkes, E.; Rabbi, I.; Akpotuzor, P.; Olaniyo, E.; Oke, E.Weed control and management are well-planned field operations carried out to prevent, remove, or limit weed infestations so the crops can grow to attain high productivity. The primary goal of weed control is to manage the vegetation in a way that will promote the growth of plants that are beneficial to humans and the suppression of unwanted plants.Item Standard Operating Procedure (SOP) for DNA extraction(Training Material, 2024-06-10) Olutegbe, A.; Nnadi, A.; Mbanjo-Edwige, G.; Rabbi, I.Deoxyribonucleic acid (DNA) extraction is an important process in molecular plant breeding. DNA extraction involves isolating DNA from the nucleus of a cell. Once purified, scientists can study individual genes, sequence the entire genome, modify sections of DNA, among others.Item Standard Operating Procedure (SOP) for seed processing, seed inventory and management in cassava(Dataset, 2024-06-10) Bakare, D.; Peteti, P.; Iluebbey, P.; Archibong, J.; John, J.; Udoh, A.; Cliff, M.; Kulakow, P.Cassava is primarily grown via stem cuttings (stakes?), but can also be propagated by seeds. Seed propagation is important to create novel gene combinations and superior cassava varieties. In cassava breeding, "seed processing" refers to the process of preparing harvested seeds, also known as "botanical seed," for sowing, distribution or storage while maintaining their viability and vigor.Item Standard Operating Procedure (SOP) for hybridization and crossing block management in cassava(Training Material, 2024-05-06) Iluebbey, P.; Kulakow, P.; Mbanjo-Edwige, G.; Bakare, D.; Peteti, P.; Toyinbo, S.; Ikpan, S.; Ogungbesan, B.; Mfam, C.Genetic variation is the basis of selection in plant breeding. Hybridization is the process of making controlled crosses between selected genotypes to provide genetic variation for stage 1 of plant breeding. This diversity created in the genetic resources provides an opportunity for plant breeders to develop new and improved cultivars with desirable characteristics.Item Standard Operating Procedures (SOP) for phenotyping data management in IITA cassava breeding programme(Dataset, 2024-06-10) Peteti, P.; Agbona, A.; Ekanem, U.; Toyinbo, S.; Dhikrullah, M.; Adetuyi, M.; Mberu, E.; Rabbi, I.; Kulakow, P.Data management involves data collection, curation, analysis, and storage to ensure accessibility to quality data. The goal of data management is to provide breeders with relevant information and tools that help to take better breeding decisions.