The potential of Rhipicephalus microplus as a vector of Ehrlichia ruminantium in West Africa

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationCentre International de Recherche-Développement sur l'Élevage en zone Subhumide, Burkina Fasoen
cg.contributor.affiliationUniversité Nazi Bonien
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationUniversité Dan Dicko Dan Koulodoen
cg.contributor.affiliationCentre de Coopération Internationale en Recherche Agronomique Pour le Développementen
cg.contributor.donorInternational Foundation of Scienceen
cg.contributor.donorInternational Mixed Laboratory on Vector-borne Diseasesen
cg.coverage.regionAfrica
cg.coverage.regionWestern Africa
cg.creator.identifierNaftaly Githaka: 0000-0003-4530-7164
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.ttbdis.2022.102117en
cg.isijournalISI Journalen
cg.issn1877-959Xen
cg.issue2en
cg.journalTicks and Tick-borne Diseasesen
cg.reviewStatusPeer Reviewen
cg.speciesRhipicephalus microplusen
cg.speciesEhrlichia ruminantiumen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriLIVESTOCKen
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 2 - Zero hungeren
cg.volume14en
dc.contributor.authorSome, M.V.en
dc.contributor.authorBiguezoton, A.S.en
dc.contributor.authorGithaka, Naftaly W.en
dc.contributor.authorAdakal, H.en
dc.contributor.authorDayo, G.-K.en
dc.contributor.authorBelem, A.en
dc.contributor.authorZoungrana, S.en
dc.contributor.authorStachurski, F.en
dc.contributor.authorChevillon, C.en
dc.date.accessioned2023-01-06T15:46:30Zen
dc.date.available2023-01-06T15:46:30Zen
dc.identifier.urihttps://hdl.handle.net/10568/126659
dc.titleThe potential of Rhipicephalus microplus as a vector of Ehrlichia ruminantium in West Africaen
dcterms.abstractHeartwater, or cowdriosis, is a virulent tick-borne rickettsial disease of ruminants caused by Ehrlichia ruminantium, biologically transmitted by Amblyomma species (A. variegatum in West Africa). In West Africa, this bacterium was recently reported to naturally infect the invasive cattle tick, Rhipicephalus microplus (Rm) through trans-ovarian transmission from replete adult females to offspring. A ‘sheep-tick-sheep’ cycle was set up to determine whether feeding the progeny of these ticks on naïve sheep could lead to infection, and to compare clinical outcomes resulting from this transmission with those observed following infection by the natural A. variegatum (Av) vector. Using local strains of ticks (KIMINI-Rm and KIMINI-Av) and of E. ruminantium (BK242), we recorded, using the PCR technique, the presence of bacterial DNA in ticks (larvae for Av and females for Rm) engorged on sheep inoculated by BK242-infected blood. The bacterial DNA was also detected in the next stages of the lifecycle of R. microplus (eggs and larvae), and in sheep infested either by those R. microplus larvae or by A. variegatum nymphs moulted from larvae engorged on blood-inoculated sheep. Bacterial infection in these sheep was demonstrated by detecting antibodies to E. ruminantium using the MAP1-B ELISA and by isolation of the bacterium on cell culture from blood. The sequences of PCS20 gene detected in ticks and sheep were identical to that of the BK242 strain. Our results confirm that R. microplus can acquire and transmit E. ruminantium to the next stage. However, this transmission resulted in a mild subclinical disease whereas severe clinical disease was observed in sheep infested by A. variegatum infected nymphs, suggesting differences in the tick/bacteria relationship. Future studies will focus on replicating these findings with ticks of different isolates and life stages to determine if R. microplus is playing a role in the epidemiology of heartwater in West Africa. Additionally, studies will investigate whether sheep that are seropositive due to infestation by E. ruminantium-infected R. microplus are subsequently protected against heartwater. Such data will add to our understanding of the possible impact of R. microplus in areas where it has become recently established.en
dcterms.accessRightsOpen Access
dcterms.audienceAcademicsen
dcterms.audienceScientistsen
dcterms.bibliographicCitationSome, M.V., Biguezoton, A.S., Githaka, N., Adakal, H., Dayo, G.-K., Belem, A., Zoungrana, S., Stachurski, F. and Chevillon, C. 2023. The potential of Rhipicephalus microplus as a vector of Ehrlichia ruminantium in West Africa. Ticks and Tick-borne Diseases 14(2): 102117.en
dcterms.extent102117en
dcterms.issued2023-03
dcterms.languageen
dcterms.licenseCC-BY-NC-ND-4.0
dcterms.publisherElsevieren
dcterms.subjectanimal diseasesen
dcterms.subjectlivestocken
dcterms.subjectheartwateren
dcterms.subjectinfectious diseasesen
dcterms.subjectmicrobiologyen
dcterms.subjectparasitologyen
dcterms.typeJournal Article

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