A multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenya

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cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationSwedish University of Agricultural Sciencesen
cg.contributor.affiliationUppsala Universityen
cg.contributor.affiliationColorado State Universityen
cg.contributor.affiliationKansas State Universityen
cg.contributor.affiliationFreie Universität Berlinen
cg.contributor.affiliationUnited States Department of Agricultureen
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.donorDepartment for International Development, United Kingdomen
cg.contributor.donorEconomic and Social Research Council, United Kingdomen
cg.contributor.donorNatural Environment Research Council, United Kingdomen
cg.contributor.donorSwedish Research Councilen
cg.contributor.donorUnited States Department of Agricultureen
cg.coverage.countryKenya
cg.coverage.iso3166-alpha2KE
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierJohanna Lindahl: 0000-0002-1175-0398en
cg.creator.identifierMartin Wainaina: 0000-0002-4938-2543en
cg.creator.identifierDeborah Mbotha: 0000-0003-2109-6411en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.jviromet.2019.04.011en
cg.isijournalISI Journalen
cg.issn0166-0934en
cg.journalJournal of Virological Methodsen
cg.reviewStatusPeer Reviewen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriDIAGNOSTICSen
cg.subject.ilriLIVESTOCKen
cg.subject.ilriRVFen
cg.subject.ilriZOONOTIC DISEASESen
cg.volume269en
dc.contributor.authorLindahl, Johanna F.en
dc.contributor.authorRagan, I.en
dc.contributor.authorRowland, R.R.en
dc.contributor.authorWainaina, M.en
dc.contributor.authorMbotha, D.en
dc.contributor.authorWilson, W.en
dc.date.accessioned2019-04-10T14:22:28Zen
dc.date.available2019-04-10T14:22:28Zen
dc.identifier.urihttps://hdl.handle.net/10568/100729
dc.titleA multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenyaen
dcterms.abstractRift Valley fever virus (RVFV) is an important mosquito-borne pathogen with devastating impacts on agriculture and public health. With outbreaks being reported beyond the continent of Africa to the Middle East, there is great concern that RVFV will continue to spread to non-endemic areas such as the Americas and Europe. There is a need for safe and high throughput serological assays for rapid detection of RVFV during outbreaks and for surveillance. We evaluated a multiplexing fluorescence microsphere immunoassay (FMIA) for the detection of IgG and IgM antibodies in ruminant sera against the RVFV nucleocapsid Np, glycoprotein Gn, and non-structural protein NSs. Sheep and cattle sera from a region in Kenya with previous outbreaks were tested by FMIA and two commercially available competitive ELISAs (BDSL and IDvet). Our results revealed strong detection of RVFV antibodies against the Np, Gn and NSs antigen targets. Additionally, testing of samples with FMIA Np and Gn had 100% agreement with the IDvet ELISA. The targets developed in the FMIA assay provided a basis for a larger ruminant disease panel that can simultaneously screen several abortive and zoonotic pathogens.en
dcterms.accessRightsLimited Access
dcterms.audienceScientistsen
dcterms.bibliographicCitationLindahl, J.F., Ragan, I., Rowland, R.R., Wainaina, M., Mbotha, D. and Wilson, W. 2019. A multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenya. Journal of Virological Methods 269: 70–76.en
dcterms.extentp. 70-76en
dcterms.issued2019-07en
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherElsevieren
dcterms.subjectzoonosesen
dcterms.subjectimmunologyen
dcterms.subjectdiagnosisen
dcterms.subjectvirologyen
dcterms.typeJournal Article

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