A multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenya

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cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationSwedish University of Agricultural Sciencesen_US
cg.contributor.affiliationUppsala Universityen_US
cg.contributor.affiliationColorado State Universityen_US
cg.contributor.affiliationKansas State Universityen_US
cg.contributor.affiliationFreie Universität Berlinen_US
cg.contributor.affiliationUnited States Department of Agricultureen_US
cg.contributor.crpAgriculture for Nutrition and Healthen_US
cg.contributor.donorDepartment for International Development, United Kingdomen_US
cg.contributor.donorEconomic and Social Research Council, United Kingdomen_US
cg.contributor.donorNatural Environment Research Council, United Kingdomen_US
cg.contributor.donorSwedish Research Councilen_US
cg.contributor.donorUnited States Department of Agricultureen_US
cg.coverage.countryKenyaen_US
cg.coverage.iso3166-alpha2KEen_US
cg.coverage.regionAfricaen_US
cg.coverage.regionEastern Africaen_US
cg.creator.identifierJohanna Lindahl: 0000-0002-1175-0398en_US
cg.creator.identifierMartin Wainaina: 0000-0002-4938-2543en_US
cg.creator.identifierDeborah Mbotha: 0000-0003-2109-6411en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.1016/j.jviromet.2019.04.011en_US
cg.isijournalISI Journalen_US
cg.issn0166-0934en_US
cg.journalJournal of Virological Methodsen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.ilriANIMAL DISEASESen_US
cg.subject.ilriDIAGNOSTICSen_US
cg.subject.ilriLIVESTOCKen_US
cg.subject.ilriRVFen_US
cg.subject.ilriZOONOTIC DISEASESen_US
cg.volume269en_US
dc.contributor.authorLindahl, Johanna F.en_US
dc.contributor.authorRagan, I.en_US
dc.contributor.authorRowland, R.R.en_US
dc.contributor.authorWainaina, M.en_US
dc.contributor.authorMbotha, D.en_US
dc.contributor.authorWilson, W.en_US
dc.date.accessioned2019-04-10T14:22:28Zen_US
dc.date.available2019-04-10T14:22:28Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/100729en_US
dc.titleA multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenyaen_US
dcterms.abstractRift Valley fever virus (RVFV) is an important mosquito-borne pathogen with devastating impacts on agriculture and public health. With outbreaks being reported beyond the continent of Africa to the Middle East, there is great concern that RVFV will continue to spread to non-endemic areas such as the Americas and Europe. There is a need for safe and high throughput serological assays for rapid detection of RVFV during outbreaks and for surveillance. We evaluated a multiplexing fluorescence microsphere immunoassay (FMIA) for the detection of IgG and IgM antibodies in ruminant sera against the RVFV nucleocapsid Np, glycoprotein Gn, and non-structural protein NSs. Sheep and cattle sera from a region in Kenya with previous outbreaks were tested by FMIA and two commercially available competitive ELISAs (BDSL and IDvet). Our results revealed strong detection of RVFV antibodies against the Np, Gn and NSs antigen targets. Additionally, testing of samples with FMIA Np and Gn had 100% agreement with the IDvet ELISA. The targets developed in the FMIA assay provided a basis for a larger ruminant disease panel that can simultaneously screen several abortive and zoonotic pathogens.en_US
dcterms.accessRightsLimited Accessen_US
dcterms.audienceScientistsen_US
dcterms.bibliographicCitationLindahl, J.F., Ragan, I., Rowland, R.R., Wainaina, M., Mbotha, D. and Wilson, W. 2019. A multiplex fluorescence microsphere immunoassay for increased understanding of Rift Valley fever immune responses in ruminants in Kenya. Journal of Virological Methods 269: 70–76.en_US
dcterms.extentp. 70-76en_US
dcterms.issued2019-07en_US
dcterms.languageenen_US
dcterms.licenseCopyrighted; all rights reserveden_US
dcterms.publisherElsevieren_US
dcterms.subjectzoonosesen_US
dcterms.subjectimmunologyen_US
dcterms.subjectdiagnosisen_US
dcterms.subjectvirologyen_US
dcterms.typeJournal Articleen_US

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