Epidemiology of antimicrobial resistant diarrheagenic Escherichia coli pathotypes from children, livestock and food in Dagoretti South, Nairobi Kenya

Simple item page
cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationKenyatta Universityen
cg.contributor.affiliationUniversity of Copenhagenen
cg.contributor.affiliationLondon School of Hygiene and Tropical Medicineen
cg.contributor.affiliationUniversity of Liverpoolen
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.donorGates Foundationen
cg.contributor.donorForeign, Commonwealth and Development Office, United Kingdomen
cg.coverage.countryKenya
cg.coverage.iso3166-alpha2KE
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierOkumu Noah: 0000-0002-5984-6444en
cg.creator.identifierDishon Muloi: 0000-0002-6236-2280en
cg.creator.identifierArshnee Moodley: 0000-0002-6469-3948en
cg.creator.identifierElizabeth Cook: 0000-0001-6081-8363en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.ijantimicag.2024.107419en
cg.isijournalISI Journalen
cg.issn0924-8579en
cg.issue3en
cg.journalInternational Journal of Antimicrobial Agentsen
cg.reviewStatusPeer Reviewen
cg.speciesEscherichia colien
cg.subject.ilriAMRen
cg.subject.ilriHUMAN HEALTHen
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 3 - Good health and well-beingen
cg.volume65en
dc.contributor.authorOkumu, Noah O.en
dc.contributor.authorMuloi, Dishon M.en
dc.contributor.authorMoodley, Arshneeen
dc.contributor.authorOchieng, Linneten
dc.contributor.authorWatson, J.en
dc.contributor.authorKiarie, Aliceen
dc.contributor.authorNgeranwa, J.J.N.en
dc.contributor.authorCumming, O.en
dc.contributor.authorCook, Elizabeth A.J.en
dc.date.accessioned2025-01-06T09:43:49Zen
dc.date.available2025-01-06T09:43:49Zen
dc.identifier.urihttps://hdl.handle.net/10568/168543
dc.titleEpidemiology of antimicrobial resistant diarrheagenic Escherichia coli pathotypes from children, livestock and food in Dagoretti South, Nairobi Kenyaen
dcterms.abstractPeri-urban environments, characterized by dense human populations, cohabiting livestock, and complex food systems, serve as hotspots for food contamination and infectious diseases. Children aged 6-24 months are particularly vulnerable as they often encounter contaminated food and water, increasing their risk of food-borne disease, with diarrhea being a common symptom. We investigated the prevalence of antimicrobial resistance (AMR) in pathogenic <i>Escherichia coli</i> from 6-24 months-old children, their food, and cohabiting livestock, in Dagoretti South subcounty in Nairobi, Kenya. Out of 540 stool, 296 livestock feces and 859 food samples collected from 585 randomly enrolled households, 16% harbored diarrheagenic <i>E. coli</i> (DEC) pathotypes. The predominant AMR phenotypes observed were trimethoprim-sulfamethoxazole, ampicillin, and tetracycline at 53%, 48% and 41%, respectively. Diarrheagenic <i>E. coli</i> from children showed significantly higher resistance to all antibiotics compared to those from livestock and food. Overall, 30% of the 274 DEC isolates from all three sources exhibited multidrug resistance. Network analysis of AMR co-occurrence revealed two clusters: 1) ampicillin, trimethoprim-sulfamethoxazole, tetracycline, amoxicillin/clavulanic acid, and chloramphenicol, and 2) nalidixic acid, ciprofloxacin, gentamicin, and ceftriaxone. Co-resistance backbone of ampicillin-trimethoprim/sulfamethoxazole-tetracycline was significantly higher among children isolates than other hosts (χ² = 29.858, df = 2, adjusted p < 0.05). Logistic regression analysis revealed that on-site disposal of animal manure and garbage, along with a recent history of diarrhea, were significantly associated with AMR carriage in children (p < 0.05). These findings emphasize the need for One Health interventions to curb emergence and spread of AMR in these close contact populations.en
dcterms.accessRightsOpen Access
dcterms.audienceAcademicsen
dcterms.audienceScientistsen
dcterms.available2024-12-24en
dcterms.bibliographicCitationOkumu, N.O., Muloi, D.M., Moodley, A., Ochieng, L., Watson, J., Kiarie, A., Ngeranwa, J.J.N., Cumming, O. and Cook, E.A.J. 2025. Epidemiology of antimicrobial resistant diarrheagenic <i>Escherichia coli</i> pathotypes from children, livestock and food in Dagoretti South, Nairobi Kenya. International Journal of Antimicrobial Agents 65(3): 107419.en
dcterms.extent107419en
dcterms.issued2025-03en
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherElsevieren
dcterms.subjectantimicrobial resistanceen
dcterms.subjecthealthen
dcterms.typeJournal Article

Files

License bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ILRI articles in journals
a ILRI Health of Animals, People and Ecosystems theme outputs (2025–)
CGIAR Antimicrobial Resistance Hub documents