Study on single nucleotide polymorphisms of H -FABP gene in sheep
| cg.coverage.country | China | en_US |
| cg.coverage.iso3166-alpha2 | CN | en_US |
| cg.coverage.region | Asia | en_US |
| cg.coverage.region | Eastern Asia | en_US |
| cg.identifier.doi | https://doi.org/10.3724/sp.j.1005.2009.00725 | en_US |
| cg.issn | 0253-9772 | en_US |
| cg.issue | 7 | en_US |
| cg.journal | Hereditas | en_US |
| cg.subject.ilri | LIVESTOCK | en_US |
| cg.subject.ilri | GENETICS | en_US |
| cg.volume | 31 | en_US |
| dc.contributor.author | Qiao, H.Y. | en_US |
| dc.contributor.author | Zhao, Q.J. | en_US |
| dc.contributor.author | Yao, N. | en_US |
| dc.contributor.author | Lu, Y.-Z. | en_US |
| dc.contributor.author | Meng, X.-R. | en_US |
| dc.contributor.author | Guan, Wei-Jun | en_US |
| dc.contributor.author | Ma, Y.H. | en_US |
| dc.contributor.author | Han Jianlin | en_US |
| dc.date.accessioned | 2010-08-04T13:29:08Z | en_US |
| dc.date.available | 2010-08-04T13:29:08Z | en_US |
| dc.identifier.uri | https://hdl.handle.net/10568/2168 | en_US |
| dc.title | Study on single nucleotide polymorphisms of H -FABP gene in sheep | en_US |
| dcterms.abstract | PCR-SSCP and DNA sequencing approaches were applied to assess the single nucleotide polymorphisms (SNPs) and analyze the genetic polymorphisms at partial exon 2 and intron 2 of H-FABP(Heart fatty acid-binding protein)gene, and five sheep populations that comprised of Small-Tailed Han sheep (SH, 48), Ningxia Tan sheep (Tan, 121), Tan × SH F1 (23), Poll Dorset (48) and Suffolk (24) sheep were screened in this stuty. The result showed: (1)four SNPs at 981(G/A), 1014(A/C) 1019(T/C) and 1058 (-/G) and nine genotypes (AA, BB, CC, AB, AC, BC, AD, CD and BD) were detected using primer 2, the AA was the predominant genotype. A chi-square analysis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium except the Tan and Suffolk populations. Statistical analysis revealed a low polymorphism information content (PIC) in the Suffolk and Tan × SH F1 populations (PIC< 0.25) but an intermediate PIC in the remaining three populations (0.25< PIC <0.50). It meant that the fragment of H-FABP had polymorphisms, which could be used as a candidate gene associated gene with phenotypic traits like intramuscular fat content in different sheep populations. (2)Three genotypes (HH, Hh and hh) determined by a SNP at 2407(T-C) were detected using primer 4. The genotype frequencies were in the order of HH＞Hh＞hh. A chi-square analy-sis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium in the Tan and Poll Dorset populations, and the PIC values were low (PIC < 0.25). However, there was no polymorphisms in SH, Tan × SH F1 and Suffolk populations. | en_US |
| dcterms.accessRights | Limited Access | en_US |
| dcterms.bibliographicCitation | Qiao, H.-Y., Zhao, Q.-J., Yao, N., Lu, Y.-Z., Meng, X.-R., Jianlin, H., Guan, W.-J.; Ma, Y.-H. 2009. Study on single nucleotide polymorphisms of H -FABP gene in sheep. Hereditas (Beijing) 31(7):725-731. | en_US |
| dcterms.extent | p. 725-731 | en_US |
| dcterms.issued | 2009-08-14 | en_US |
| dcterms.language | zh | en_US |
| dcterms.publisher | China Science Publishing & Media Ltd. | en_US |
| dcterms.subject | genetics | en_US |
| dcterms.subject | sheep | en_US |
| dcterms.type | Journal Article | en_US |
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