Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva

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cg.creator.identifierPhilip Toye: 0000-0002-7100-2789
cg.creator.identifierVishvanath Nene: 0000-0001-7066-4169
cg.creator.identifierRichard Bishop: 0000-0002-3720-9970
cg.issn0166-6851en
cg.journalMolecular and Biochemical Parasitologyen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriDISEASE CONTROLen
cg.subject.ilriLIVESTOCKen
cg.subject.ilriVACCINESen
dc.contributor.authorToye, Philip G.en
dc.contributor.authorGobright, E.I.en
dc.contributor.authorNyanjui, J.en
dc.contributor.authorNene, Vishvanath M.en
dc.contributor.authorBishop, Richard P.en
dc.date.accessioned2013-06-11T09:22:58Zen
dc.date.available2013-06-11T09:22:58Zen
dc.identifier.urihttps://hdl.handle.net/10568/29265
dc.titleStructure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parvaen
dcterms.abstractInitial comparison of 2 polymorphic, immunodominant molecule (PIM) cDNA sequences from Theileria parva suggested that the PIM genes consist of conserved 5' and 3' termini flanking a central variable region. Further evidence is presented, based on sequence analysis, supporting this general structure for the PIM genes. Evidence is also presented for a single copy of the PIM gene per haploid genome, implying that the different versions of PIM are encoded by distinct alleles. The central variable region of the PIM allele from the T. parva (Marikebuni) stock was found to contain 13 copies of the tetrapeptide repeat Gln-Pro-Glu-Pro. Point mutations were also detected in the 5' and 3' termini of the PIM alleles, including regions recognized by the neutralizing and typing MAb. This contrasted with the high sequence conservation of the 2 introns of the genes, suggesting that the protein is undergoing rapid evolution. Sequence comparison of PIM genes from buffalo- and cattle-derived parasites supported earlier results that the parasites infecting buffaloes constitute a more heterogeneous population than those from cattle.en
dcterms.accessRightsLimited Access
dcterms.bibliographicCitationMolecular and Biochemical Parasitology;73(1-2): 165-177en
dcterms.extentp. 165-177en
dcterms.issued1995
dcterms.languageen
dcterms.subjectgenesen
dcterms.subjectantigensen
dcterms.subjecttheileria parvaen
dcterms.subjectanimal diseasesen
dcterms.subjectdiagnosisen
dcterms.typeJournal Article

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