Nontargeted metabolomics: PTFI Platform

Watkins, S.; Odenkirk, M.T.; Prenni, J.; Brinkley, S. (2025) Nontargeted metabolomics: PTFI Platform. 3 p.

The PTFI Nontargeted Metabolomics platform supports the characterization of the small molecule composition in food. This method uses solid phase extraction (SPE) to isolate small molecules for analysis by reverse phased liquid chromatography-high resolution mass spectrometry (LC-HRMS).

The PTFI Nontargeted Metabolomics platform is adapted from existing protocols used by researchers. What sets the PTFI method apart is the focus on standardization and the incorporation of a unique internal retention standard reagent which is a mixture of 33 compounds nonendogenous to food. When researchers around the world employ the same standardized protocol and utilize the same internal standard reagent the resulting data can be harmonized and becomes comparable – allowing us to build a scalable data resource.