Comparative evaluation of LAMP, qPCR, conventional PCR, and ELISA to detect ralstonia solanacearum in Kenyan potato fields

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cg.authorship.typesCGIAR multi-centreen
cg.contributor.affiliationEgerton Universityen
cg.contributor.affiliationJomo Kenyatta University of Agriculture and Technologyen
cg.contributor.affiliationCornell Universityen
cg.contributor.affiliationInternational Potato Centeren
cg.contributor.crpRoots, Tubers and Bananasen
cg.contributor.donorSyngenta Foundation for Sustainable Agricultureen
cg.contributor.donorBill & Melinda Gates Foundationen
cg.contributor.donorDepartment for International Development, United Kingdomen
cg.contributor.donorSwedish International Development Cooperation Agencyen
cg.contributor.donorDepartment of Foreign Affairs and Trade, Australiaen
cg.coverage.countryKenyaen
cg.coverage.iso3166-alpha2KEen
cg.coverage.regionAfricaen
cg.coverage.regionEastern Africaen
cg.creator.identifierLilian A. Okiro: 0000-0002-6531-4604en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1094/pdis-03-18-0489-reen
cg.isijournalISI Journalen
cg.issn0191-2917en
cg.issue5en
cg.journalPlant Diseaseen
cg.reviewStatusPeer Reviewen
cg.subject.cipCROP PROTECTIONen
cg.subject.cipPOTATO AGRI-FOOD SYSTEMSen
cg.subject.cipPOTATOESen
cg.volume103en
dc.contributor.authorOkiro, L.A.en
dc.contributor.authorTancos, M.A.en
dc.contributor.authorNyanjom, S.G.en
dc.contributor.authorSmart, C.D.en
dc.contributor.authorParker, M.en
dc.date.accessioned2019-07-08T21:12:05Zen
dc.date.available2019-07-08T21:12:05Zen
dc.identifier.urihttps://hdl.handle.net/10568/102098
dc.titleComparative evaluation of LAMP, qPCR, conventional PCR, and ELISA to detect ralstonia solanacearum in Kenyan potato fieldsen
dcterms.abstractBacterial wilt caused by Ralstonia solanacearum is considered among the most damaging diseases of potato in Sub-Saharan Africa and the most significant biotic constraint of potato production alongside late blight. Unlike late blight, which can be managed by chemical means, R. solanacearum can only be managed through cultural methods and clean seed. Laboratory testing to certify seed before planting is required to confirm the absence of the pathogen in Kenya. A loop-mediated isothermal amplification (LAMP) assay was developed using the UDP-(3-O-acyl)-N-acetylglucosamine deacetylase gene (IpxC) to screen seed potato for R. solanacearum strains. The assay was assessed using DNA extracted from R. solanacearum and other soil and potato pathogens to demonstrate specificity and sensitivity. The LAMP assay was validated using field samples from different potato growing regions of Kenya collected over two growing seasons and compared with established nucleic acid and protein-based assays. The IpxC LAMP assay was found to be specific and sensitive to R. solanacearum, detecting as low as 2.5 pg/µl of R. solanacearum DNA. Of the 47 potentially infected field samples collected, both IpxC LAMP and quantitative polymerase chain reaction (PCR) detected R. solanacearum DNA in 90% of the samples, followed by conventional PCR (86%) and ELISA (75%). This IpxC LAMP assay is a promising diagnostic tool to rapidly screen for R. solanacearum in seed potato with high sensitivity in Kenya.en
dcterms.accessRightsOpen Accessen
dcterms.audienceScientistsen
dcterms.bibliographicCitationOkiro, L.A.; Tancos, M.A.; Nyanjom, S.G.; Smart, C.D.; Parker, M.L. 2019. Comparative evaluation of LAMP, qPCR, conventional PCR, and ELISA to detect ralstonia solanacearum in Kenyan potato fields. Plant Disease. ISSN: 0191-2917. 103:5. pp. 959-965.en
dcterms.extent959-965en
dcterms.issued2019-05en
dcterms.languageenen
dcterms.licenseCC-BY-4.0en
dcterms.publisherScientific Societiesen
dcterms.subjectpotatoesen
dcterms.subjectralstonia solanacearumen
dcterms.subjectpcren
dcterms.typeJournal Articleen

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