Genomic organization of the bovine class II MHC studied with field inversion gel electrophoresis

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cg.issn0268-9146en
cg.journalAnimal Geneticsen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriDISEASE CONTROLen
cg.subject.ilriLIVESTOCKen
cg.subject.ilriGENETICSen
dc.contributor.authorBensaid, A.M.en
dc.contributor.authorYoung, J.R.en
dc.contributor.authorKaushal, A.en
dc.contributor.authorTeale, A.J.en
dc.date.accessioned2013-05-06T07:00:13Zen
dc.date.available2013-05-06T07:00:13Zen
dc.identifier.urihttps://hdl.handle.net/10568/28245
dc.titleGenomic organization of the bovine class II MHC studied with field inversion gel electrophoresisen
dcterms.abstractIn a study Bovine DNA was prepared from a Theileria parva-transformed lymphobastoid cell line derived from the PBM of an heterozygons Boran (Bos indicus) steer. It was observed that linkage between the bovine DR alpha and DR beta subregions on the basis that a 1-2 Mbp Sfi1 and a 0.6 Mbp not 1 fragment hybridized with both probes. In view of the fact that these probes revealed, in addition, urigne extra bands with both enzymes, it is concluded that they may be considered locus-specific in cattle. The DP beta cDNA hybridized with a 0.32 Mbp Sfi1 and 0.6 and 0.1 Mbp Not1 fragments. Only the highest molecular weight fragment seems to be common with the DR region. Sight differences in mobility between the Sfi1 DR beta and DP beta bands (0.3 versus 0.32 Mbp) have been observed. The result suggest that the bovine MHC spans more than 3 MbThe class II region is composed of tightly linked DR alpha and DR beta subregions.en
dcterms.accessRightsLimited Access
dcterms.bibliographicCitationAnimal Genetics;19: 42-44en
dcterms.extentp. 42-44en
dcterms.issued1988
dcterms.languageen
dcterms.subjectgenomicsen
dcterms.subjectbovinaeen
dcterms.subjectmajor histocompatibility complexen
dcterms.subjectelectrophoresisen
dcterms.typeJournal Article

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