Genomic organization of the bovine class II MHC studied with field inversion gel electrophoresis

cg.issn0268-9146en_US
cg.journalAnimal Geneticsen_US
cg.subject.ilriANIMAL DISEASESen_US
cg.subject.ilriDISEASE CONTROLen_US
cg.subject.ilriLIVESTOCKen_US
cg.subject.ilriGENETICSen_US
dc.contributor.authorBensaid, A.M.en_US
dc.contributor.authorYoung, J.R.en_US
dc.contributor.authorKaushal, A.en_US
dc.contributor.authorTeale, A.J.en_US
dc.date.accessioned2013-05-06T07:00:13Zen_US
dc.date.available2013-05-06T07:00:13Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/28245en_US
dc.titleGenomic organization of the bovine class II MHC studied with field inversion gel electrophoresisen_US
dcterms.abstractIn a study Bovine DNA was prepared from a Theileria parva-transformed lymphobastoid cell line derived from the PBM of an heterozygons Boran (Bos indicus) steer. It was observed that linkage between the bovine DR alpha and DR beta subregions on the basis that a 1-2 Mbp Sfi1 and a 0.6 Mbp not 1 fragment hybridized with both probes. In view of the fact that these probes revealed, in addition, urigne extra bands with both enzymes, it is concluded that they may be considered locus-specific in cattle. The DP beta cDNA hybridized with a 0.32 Mbp Sfi1 and 0.6 and 0.1 Mbp Not1 fragments. Only the highest molecular weight fragment seems to be common with the DR region. Sight differences in mobility between the Sfi1 DR beta and DP beta bands (0.3 versus 0.32 Mbp) have been observed. The result suggest that the bovine MHC spans more than 3 MbThe class II region is composed of tightly linked DR alpha and DR beta subregions.en_US
dcterms.accessRightsLimited Accessen_US
dcterms.bibliographicCitationAnimal Genetics;19: 42-44en_US
dcterms.extentp. 42-44en_US
dcterms.issued1988en_US
dcterms.languageenen_US
dcterms.subjectgenomicsen_US
dcterms.subjectbovinaeen_US
dcterms.subjectmajor histocompatibility complexen_US
dcterms.subjectelectrophoresisen_US
dcterms.typeJournal Articleen_US

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