One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins

Loading...
Thumbnail Image

Date Issued

Date Online

2000-05-12

Language

en

Review Status

Peer Review

Access Rights

Limited Access Limited Access

Share

Citation

Machuka, J. & Okeola, O. (2000). One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins. Journal of Agricultural and Food Chemistry, 48(6), 2296-2299.

Permanent link to cite or share this item

External link to download this item

Abstract/Description

Seed proteins were extracted from the African yam bean (AYB; Sphenostylis stenocarpa), an underutilized West African food legume. One- and two-dimensional polyacrylamide gel electrophoresis was then used to analyze the albumin fraction, galactose-specific lectins purified on immobilized galactose−Sepharose 4B, and abundant non-lectin seed proteins left over following affinity chromatography. N-terminal sequencing of prominently resolved polypetide bands led to identification of proteins having sequence homology with characterized legume seed proteins, namely, mung bean seed albumin, pea α-fucosidase, soybean Kunitz-type trypsin inhibitor, an endochitinase, pea pathogenesis-related protein, and/or cowpea seed storage proteins. Minor lectin-like proteins lacking hemagglutinating activity against rabbit and human erythrocytes were also identified. Because proteins such as protease inhibitors, chitinases, pathogenesis-related proteins, and lectins are known to have antimetabolic effects, the findings from this study may have relevance in the acceptability, adoption, and utilization of AYB as human food.

AGROVOC Keywords
Countries
Organizations Affiliated to the Authors