One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins
| cg.authorship.types | CGIAR single centre | en |
| cg.contributor.affiliation | International Institute of Tropical Agriculture | en |
| cg.coverage.country | Nigeria | |
| cg.coverage.iso3166-alpha2 | NG | |
| cg.coverage.region | Africa | |
| cg.coverage.region | Western Africa | |
| cg.identifier.doi | https://doi.org/10.1021/jf990800x | en |
| cg.isijournal | ISI Journal | en |
| cg.issn | 0021-8561 | en |
| cg.issn | 1520-5118 | en |
| cg.issue | 6 | en |
| cg.journal | Journal of Agricultural and Food Chemistry | en |
| cg.reviewStatus | Peer Review | en |
| cg.subject.iita | GRAIN LEGUMES | en |
| cg.subject.iita | FOOD SECURITY | en |
| cg.subject.iita | FOOD SYSTEMS | en |
| cg.subject.iita | PLANT DISEASES | en |
| cg.volume | 48 | en |
| dc.contributor.author | Machuka, J. | en |
| dc.contributor.author | Okeola, O. | en |
| dc.date.accessioned | 2019-03-03T05:53:46Z | en |
| dc.date.available | 2019-03-03T05:53:46Z | en |
| dc.identifier.uri | https://hdl.handle.net/10568/99870 | |
| dc.title | One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins | en |
| dcterms.abstract | Seed proteins were extracted from the African yam bean (AYB; Sphenostylis stenocarpa), an underutilized West African food legume. One- and two-dimensional polyacrylamide gel electrophoresis was then used to analyze the albumin fraction, galactose-specific lectins purified on immobilized galactose−Sepharose 4B, and abundant non-lectin seed proteins left over following affinity chromatography. N-terminal sequencing of prominently resolved polypetide bands led to identification of proteins having sequence homology with characterized legume seed proteins, namely, mung bean seed albumin, pea α-fucosidase, soybean Kunitz-type trypsin inhibitor, an endochitinase, pea pathogenesis-related protein, and/or cowpea seed storage proteins. Minor lectin-like proteins lacking hemagglutinating activity against rabbit and human erythrocytes were also identified. Because proteins such as protease inhibitors, chitinases, pathogenesis-related proteins, and lectins are known to have antimetabolic effects, the findings from this study may have relevance in the acceptability, adoption, and utilization of AYB as human food. | en |
| dcterms.accessRights | Limited Access | |
| dcterms.available | 2000-05-12 | |
| dcterms.bibliographicCitation | Machuka, J. & Okeola, O. (2000). One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins. Journal of Agricultural and Food Chemistry, 48(6), 2296-2299. | en |
| dcterms.extent | pp. 2296-2299 | en |
| dcterms.issued | 2000-06-01 | |
| dcterms.language | en | |
| dcterms.publisher | American Chemical Society | en |
| dcterms.subject | legumes | en |
| dcterms.subject | foods | en |
| dcterms.subject | proteins | en |
| dcterms.subject | pathogenesis | en |
| dcterms.type | Journal Article |