Improved sample selection and preparation methods for sampling plans used to facilitate rapid and reliable estimation of aflatoxin in chicken feed

Simple item page
cg.authorship.typesCGIAR and developing country instituteen_US
cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationKenya Agricultural and Livestock Research Organizationen_US
cg.contributor.affiliationKenyatta Universityen_US
cg.contributor.affiliationNorth Carolina State Universityen_US
cg.contributor.affiliationHygiena LLCen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationUniversity of Greenwichen_US
cg.contributor.affiliationSwedish University of Agricultural Sciencesen_US
cg.contributor.affiliationUppsala Universityen_US
cg.contributor.crpAgriculture for Nutrition and Healthen_US
cg.contributor.donorGovernment of Kenyaen_US
cg.creator.identifierDelia Grace: 0000-0002-0195-9489en_US
cg.creator.identifierJohanna Lindahl: 0000-0002-1175-0398en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.3390/toxins13030216en_US
cg.isijournalISI Journalen_US
cg.issn2072-6651en_US
cg.issue3en_US
cg.journalToxinsen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.ilriAFLATOXINSen_US
cg.subject.ilriCHICKENSen_US
cg.subject.ilriFEEDSen_US
cg.subject.ilriPOULTRYen_US
cg.subject.impactAreaNutrition, health and food securityen_US
cg.subject.sdgSDG 2 - Zero hungeren_US
cg.volume13en_US
dc.contributor.authorKibugu, J.en_US
dc.contributor.authorMdachi, R.en_US
dc.contributor.authorMunga, L.en_US
dc.contributor.authorMburu, D.en_US
dc.contributor.authorWhitaker, T.en_US
dc.contributor.authorThu P. Huynhen_US
dc.contributor.authorGrace, Deliaen_US
dc.contributor.authorLindahl, Johanna F.en_US
dc.date.accessioned2021-03-18T07:13:17Zen_US
dc.date.available2021-03-18T07:13:17Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/113041en_US
dc.titleImproved sample selection and preparation methods for sampling plans used to facilitate rapid and reliable estimation of aflatoxin in chicken feeden_US
dcterms.abstractAflatoxin B1 (AFB1), a toxic fungal metabolite associated with human and animal diseases, is a natural contaminant encountered in agricultural commodities, food and feed. Heterogeneity of AFB1 makes risk estimation a challenge. To overcome this, novel sample selection, preparation and extraction steps were designed for representative sampling of chicken feed. Accuracy, precision, limits of detection and quantification, linearity, robustness and ruggedness were used as performance criteria to validate this modification and Horwitz function for evaluating precision. A modified sampling protocol that ensured representativeness is documented, including sample selection, sampling tools, random procedures, minimum size of field-collected aggregate samples (primary sampling), procedures for mass reduction to 2 kg laboratory (secondary sampling), 25 g test portion (tertiary sampling) and 1.3 g analytical samples (quaternary sampling). The improved coning and quartering procedure described herein (for secondary and tertiary sampling) has acceptable precision, with a Horwitz ratio (HorRat = 0.3) suitable for splitting of 25 g feed aliquots from laboratory samples (tertiary sampling). The water slurring innovation (quaternary sampling) increased aflatoxin extraction efficiency to 95.1% through reduction of both bias (−4.95) and variability of recovery (1.2–1.4) and improved both intra-laboratory precision (HorRat = 1.2–1.5) and within-laboratory reproducibility (HorRat = 0.9–1.3). Optimal extraction conditions are documented. The improved procedure showed satisfactory performance, good field applicability and reduced sample analysis turnaround time.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceScientistsen_US
dcterms.available2021-03-16en_US
dcterms.bibliographicCitationKibugu, J., Mdachi, R., Munga, L., Mburu, D., Whitaker, T., Thu P. Huynh, Grace, D. and Lindahl, J.F. 2021. Improved sample selection and preparation methods for sampling plans used to facilitate rapid and reliable estimation of aflatoxin in chicken feed. Toxins 13(3): 216.en_US
dcterms.extent216en_US
dcterms.issued2021-03-16en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.publisherMDPIen_US
dcterms.subjectaflatoxinsen_US
dcterms.subjectfeedsen_US
dcterms.subjectpoultryen_US
dcterms.subjectchickensen_US
dcterms.subjecttoxicologyen_US
dcterms.typeJournal Articleen_US

Files

License bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ILRI articles in journals
CRP A4NH outputs
ILRI A4NH food safety flagship outputs (2012-2021)
ILRI animal and human health program outputs (2017-2024)