Identification and functional analysis of ferritin 2 from the Taiga tick Ixodes persulcatus Schulze

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cg.authorship.typesCGIAR and developing country instituteen_US
cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationHokkaido Universityen_US
cg.contributor.affiliationUniversidade Federal do Rio Grande do Sulen_US
cg.contributor.affiliationUniversidade Federal do Rio de Janeiroen_US
cg.contributor.crpLivestocken_US
cg.contributor.donorJapan Society for the Promotion of Scienceen_US
cg.contributor.donorJapan Agency for Medical Research and Developmenten_US
cg.contributor.donorCoordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazilen_US
cg.creator.identifierNaftaly Githaka: 0000-0003-4530-7164en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.1016/j.ttbdis.2020.101547en_US
cg.isijournalISI Journalen_US
cg.issn1877-959Xen_US
cg.issue6en_US
cg.journalTicks and Tick-borne Diseasesen_US
cg.reviewStatusPeer Reviewen_US
cg.speciesIxodes persulcatusen_US
cg.subject.ilriANIMAL HEALTHen_US
cg.subject.ilriRESEARCHen_US
cg.volume11en_US
dc.contributor.authorGithaka, Naftaly W.en_US
dc.contributor.authorKonnai, S.en_US
dc.contributor.authorIsezaki, M.en_US
dc.contributor.authorGoto, S.en_US
dc.contributor.authorXavier, M.A.en_US
dc.contributor.authorFujisawa, S.en_US
dc.contributor.authorYamada, S.en_US
dc.contributor.authorOkagawa, T.en_US
dc.contributor.authorMaekawa, N.en_US
dc.contributor.authorLogullo, C.en_US
dc.contributor.authorSilva Vaz, I. daen_US
dc.contributor.authorMurata, S.en_US
dc.contributor.authorOhashi, K.en_US
dc.date.accessioned2020-10-09T16:09:14Zen_US
dc.date.available2020-10-09T16:09:14Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/109799en_US
dc.titleIdentification and functional analysis of ferritin 2 from the Taiga tick Ixodes persulcatus Schulzeen_US
dcterms.abstractFerritin 2 (FER2) is an iron storage protein, which has been shown to be critical for iron homeostasis during blood feeding and reproduction in ticks and is therefore suitable as a component for anti-tick vaccines. In this study, we identified the FER2 of Ixodes persulcatus, a major vector for zoonotic diseases such as Lyme borreliosis and tick-borne relapsing fever in Japan, and investigated its functions. Ixodes persulcatus-derived ferritin 2 (Ip-FER2) showed concentration-dependent iron-binding ability and high amino acid conservation, consistent with FER2s of other tick species. Vaccines containing the recombinant Ip-FER2 elicited a significant reduction of the engorgement weight of adult I. persulcatus. Interestingly, the reduction of engorgement weight was also observed in Ixodes ovatus, a sympatric species of I. persulcatus. In silico analyses of FER2 sequences of I. persulcatus and other ticks showed a greater similarity with I. scapularis and I. ricinus and lesser similarity with Hyalomma anatolicum, Haemaphysalis longicornis, Rhipicephalus microplus, and R. appendiculatus. Moreover, it was observed that the tick FER2 sequences possess conserved regions within the primary structures, and in silico epitope mapping analysis revealed that antigenic regions were also conserved, particularly among Ixodes spp ticks. In conclusion, the data support further protective tick vaccination applications using the Ip-FER2 antigens identified herein.en_US
dcterms.accessRightsLimited Accessen_US
dcterms.audienceScientistsen_US
dcterms.bibliographicCitationGithaka, N.W., Konnai, S., Isezaki, M., Goto, S., Xavier, M.A., Fujisawa, S., Yamada, S., Okagawa, T., Maekawa, N., Logullo, C., Silva Vaz Jr., I. da, Murata, S. and Ohashi, K. 2020. Identification and functional analysis of ferritin 2 from the Taiga tick Ixodes persulcatus Schulze. Ticks and Tick-borne Diseases 11(6): 101547.en_US
dcterms.issued2020-11en_US
dcterms.languageenen_US
dcterms.licenseCopyrighted; all rights reserveden_US
dcterms.publisherElsevieren_US
dcterms.subjectvectorsen_US
dcterms.subjectanimal healthen_US
dcterms.subjectresearchen_US
dcterms.typeJournal Articleen_US

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