Improvement of recovery cycle of cryopreserved potato shoot tips – Small changes, but huge impact

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cg.authorship.typesCGIAR single centreen
cg.contributor.affiliationInternational Potato Centeren
cg.contributor.donorGlobal Crop Diversity Trusten
cg.creator.identifierRainer Vollmer: 0000-0001-8428-2241
cg.creator.identifierJaneth Espirilla: 0000-0002-8786-0606
cg.creator.identifierMario Castro CHaves: 0000-0002-0503-6454
cg.creator.identifierVania Azevedo: 0000-0003-1664-1382
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.cryobiol.2022.11.044en
cg.isijournalISI Journalen
cg.issn1090-2392en
cg.journalCryobiologyen
cg.reviewStatusPeer Reviewen
cg.subject.cipPOTATO AGRI-FOOD SYSTEMSen
cg.subject.cipPOTATOESen
cg.volume109en
dc.contributor.authorVollmer, R.en
dc.contributor.authorEspirilla, J.en
dc.contributor.authorVillagaray, R.en
dc.contributor.authorCastro, M.en
dc.contributor.authorCardenas, J.en
dc.contributor.authorPineda, S.en
dc.contributor.authorAzevedo, V.C.R.en
dc.date.accessioned2023-03-23T16:46:48Zen
dc.date.available2023-03-23T16:46:48Zen
dc.identifier.urihttps://hdl.handle.net/10568/129750
dc.titleImprovement of recovery cycle of cryopreserved potato shoot tips – Small changes, but huge impacten
dcterms.abstractImprovement and optimization of cryopreservation protocols for its reliable large-scale application is a time-consuming process. The development of robust and efficient protocols implicates the assessment of a high number of diverse genotypes before results can be extrapolated on complete collections. To simplify and improve the recovery cycle of potato shoot tips cryopreserved with the droplet-vitrification method, it was assessed if placing shoot tips after thawing for 9 days on culture medium with normal and stable sucrose concentration of 0.073 M (in darkness), can increase the viability rate, compared to a stepwise decrease of the sucrose concentration from 0.3 M to 0.2 M to 0.1M and 0.073 M during the initial phase of the recovery cycle (control treatment). The experiment was performed with 68 diverse potato landraces which showed a significantly higher recovery rate of 69.8 % when shoot tips were recovered on culture medium with a stable sucrose concentration of 0.073 M, compared to the control treatment (57.9 %). The difference in recovery rates was confirmed during routine cryopreservation: a set of 806 accessions recovered with the control treatment showed an average recovery rate of 60.9 % (years 2018-19), while 1158 accessions processed with the new protocol had an average recovery rate of 75.1 % (2019-now). Currently, CIP conserves 4017 virus-free potato accessions in its cryobank, which represent about 83% of the in vitro collection (4851 accessions). Based on a high experimental sample size of 68 tested accessions (1.4 % of population) robust and reliable results about the cryo-response of the whole collection were obtained.en
dcterms.accessRightsLimited Access
dcterms.audienceAcademicsen
dcterms.audienceCGIARen
dcterms.audienceDevelopment Practitionersen
dcterms.audienceDonorsen
dcterms.audienceExtensionen
dcterms.audienceFarmersen
dcterms.audienceGeneral Publicen
dcterms.audienceNGOsen
dcterms.audiencePolicy Makersen
dcterms.audienceScientistsen
dcterms.available2022-12
dcterms.bibliographicCitationVollmer, R.; Espirilla, J.; Villagaray, R.; Castro, M.; Cárdenas, J.; Pineda, S.; Azevedo, V. 2022. Improvement of recovery cycle of cryopreserved potato shoot tips – Small changes, but huge impact. Cryobiology, 109, 13–14. https://doi.org/10.1016/j.cryobiol.2022.11.044en
dcterms.extent13-14en
dcterms.issued2022-12
dcterms.languagees
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherElsevieren
dcterms.subjectcryopreservationen
dcterms.subjectpotatoesen
dcterms.typeJournal Article

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