SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationChinese Academy of Tropical Agricultural Sciencesen
cg.contributor.affiliationHainan Universityen
cg.contributor.affiliationInternational Center for Tropical Agricultureen
cg.contributor.donorNational Natural Science Foundation of Chinaen
cg.contributor.donorCGIAR Trust Funden
cg.contributor.initiativeAccelerated Breedingen
cg.creator.identifierIdupulapati M. Rao: 0000-0002-8381-9358en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.3389/fpls.2022.1027551en
cg.isijournalISI Journalen
cg.issn1664-462Xen
cg.journalFrontiers in Plant Scienceen
cg.reviewStatusPeer Reviewen
cg.subject.actionAreaResilient Agrifood Systemsen
cg.subject.alliancebiovciatPLANT BREEDINGen
cg.subject.impactAreaNutrition, health and food securityen
cg.subject.sdgSDG 1 - No povertyen
cg.subject.sdgSDG 2 - Zero hungeren
cg.volume13en
dc.contributor.authorZou, Xiaoyanen
dc.contributor.authorHuang, Ruien
dc.contributor.authorWang, Linjieen
dc.contributor.authorWang, Guihuaen
dc.contributor.authorMiao, Yeen
dc.contributor.authorRao, Idupulapati M.en
dc.contributor.authorLiu, Guodaoen
dc.contributor.authorChen, Zhijianen
dc.date.accessioned2023-01-14T18:53:02Zen
dc.date.available2023-01-14T18:53:02Zen
dc.identifier.urihttps://hdl.handle.net/10568/127145
dc.titleSgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensisen
dcterms.abstractTransporters belonging to the natural resistance-associated macrophage protein (Nramp) family play important roles in metal uptake and homeostasis. Although Nramp members have been functionally characterized in plants, the role of Nramp in the important tropical forage legume Stylosanthes guianensis (stylo) is largely unknown. This study aimed to determine the responses of Nramp genes to metal stresses and investigate its metal transport activity in stylo. Five SgNramp genes were identified from stylo. Expression analysis showed that SgNramp genes exhibited tissue preferential expressions and diverse responses to metal stresses, especially for manganese (Mn), suggesting the involvement of SgNramps in the response of stylo to metal stresses. Of the five SgNramps, SgNramp1 displayed the highest expression in stylo roots. A close correlation between SgNramp1 expression and root Mn concentration was observed among nine stylo cultivars under Mn limited condition. The higher expression of SgNramp1 was correlated with a high Mn uptake in stylo. Subsequent subcellular localization analysis showed that SgNramp1 was localized to the plasma membrane. Furthermore, heterologous expression of SgNramp1 complemented the phenotype of the Mn uptake-defective yeast (Saccharomyces cerevisiae) mutant Dsmf1. Mn concentration in the yeast cells expressing SgNramp1 was higher than that of the empty vector control, suggesting the transport activity of SgNramp1 for Mn in yeast. Taken together, this study reveals that SgNramp1 is a plasma membrane–localized transporter responsible for Mn uptake in stylo.en
dcterms.accessRightsOpen Accessen
dcterms.available2022-10-06en
dcterms.bibliographicCitationZou, X.; Huang, R.; Wang, L.; Wang, G.; Miao, Y.; Rao, I.; Liu, G.; Chen, Z. (2022) SgNramp1, a plasma membrane-localized transporter, involves in manganese uptake in Stylosanthes guianensis. Frontiers in Plant Science 13:1027551. ISSN: 1664-462Xen
dcterms.issued2022-10-06en
dcterms.languageenen
dcterms.licenseCC-BY-4.0en
dcterms.publisherFrontiers Mediaen
dcterms.subjecthomeostasisen
dcterms.subjectgene expressionen
dcterms.subjectstylosanthes guianensisen
dcterms.subjecttrace elementsen
dcterms.subjectpcren
dcterms.typeJournal Articleen

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